197 140

Cited 0 times in

한국에서 분리된 plasmid-mediated AmpC β-lactamase 생성 Escherichia coli와 Klebsiella pneumoniae의 빈도와 유전자형의 분포

Other Titles
 Occurrence and Genotypic Distributions of Plasmid-Mediated AmpC β-Lactamase-producing Escherichia coil and Klebsiella pneumoniae in korea 
Authors
 송원근  ;  김재석  ;  김미나  ;  김의종  ;  박연준  ;  용동은  ;  이경원  ;  이위교  ;  장석훈  ;  이규만 
Citation
 KOREAN JOURNAL OF LABORATORY MEDICINE , Vol.22(6) : 410-416, 2002 
Journal Title
 KOREAN JOURNAL OF LABORATORY MEDICINE 
ISSN
 1598-6535 
Issue Date
2002
Keywords
Plasmid-mediated AmpC -lactamase ; Escherichia coli ; Klebsiella pneumoniae ; Modified Hodge test ; Multiplex PCR ; Korea
Abstract
Background :Plasmid-mediated AmpC -lactamases (PABL) are cephalosporinases that confer resistance to a wide variety of -lactam drugs and that may thereby create serious therapeutic problems. The PABL-producing organisms are a major concern in nosocomial infections and should therefore be monitored in surveillance studies. Although reported with increasing frequency in Korea, the occurrence and genotypic distributions of PABL in Escherichia coli and Klebsiella pneumoniae remain unknown. Method :We tested a total of 911 consecutive, nonduplicate isolates of E. coli and K. pneumoniae at 12 university hospitals and a commercial laboratory in Korea. Antimicrobial susceptibilities were tested using the disk diffusion method. PABL production was determined by the modified Hodge test and multiplex PCR. The PCR differentiated the six PABL-specific families in E. coli and K. pneumoniae. Result :Overall, 110 (12.1%) yielded cefoxitin non-susceptible isolates and that 28 (3.1%) demonstrated PABL producers by multiplex PCR. Based on the species, of 544 E. coli and 367 K. pneumoniae isolates tested, 8 (1.5%) and 20 (5.4%), respectively, demonstrated PABL producers. The genotypes of PCR amplification showed that the MOX, DHA, and CIT family were harbored by 4, 2, and 2 of 8 PABL-producing E. coli, and the DHA, MOX, and EBC family were harbored by 13, 6, and 1 of 20 PABL-producing K. pneumoniae isolates, respectively. Conclusion :These data confirm that the occurrence of PABL-producing E. coli and K. pneumoniae is relatively high and the kinds of genotypes are variously distributed in Korea.
Files in This Item:
T200205491.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143841
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse