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모체의 말초 혈액에 존재하는 태아 세포 분리에 의한 새로운 기형아 진단 : Microdissection에 의한 CGH 기법의 사용

Other Titles
 A New Prenatal Diagnosis of Fetal Cells Isolation from Maternal Peripheral Blood - Using Comparative Genomic Hybridization by Microdissection 
 양영호  ;  김성훈  ;  김세광  ;  박용원  ;  조재성  ;  김인규  ;  최종락  ;  김미순 
 Korean Journal of Obstetrics and Gynecology (대한산부인과학회잡지), Vol.45(2) : 213-219, 2002 
Journal Title
 Korean Journal of Obstetrics and Gynecology (대한산부인과학회잡지) 
Issue Date
Prenatal diagnosis ; Fetal nRBCs ; Microdissection ; Comparative genomic hybridization
Objective: The objective of this study was to determine the clinical use of CGH (comparative genomic hybridization) for detection of fetal aneuploidy from fetal cells (nucleated red blood cells, nRBCs) isolated Methods: Maternal peripheral venous blood sample was collected and treated by heparin. Triple density gradient centrifugation, and MACS (magnetic activated cell sorting) using CD45 and CD 71 were used to isolated the fetal nRBCs. With microdissection, DOP (degenerate oligonucleotide primed)-PCR (polymerase chain reaction), and nick translation, CGH was performed. Results: Fetal nRBCs were successfully isolated from maternal peripheral blood. After microdissection of fetal nRBCs, DOP-PCR. and nick translation, DNA size was suitable for hybridization. In CGH analysis, we can confirm normal female and trisomy 21 male fetus. Conclusion: Prenatal diagnosis from fetal cells in maternal peripheral blood by comparative genomic hybridization shows clinical promise in terms of speed, accuracy, and non-invasiveness. To enable widespread use of this method, further studies involving many cases are warrented.
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1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sei Kwang(김세광)
Park, Yong Won(박용원)
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