silica ; reactive oxygen species ; peroxiredoxin ; protein degradation ; calcium
Abstract
Silica has been known to be a factor in acute cell injury and chronic pulmonary fibrosis. To
date, many studies have emphasized the reactive oxygen species (ROS) as a primary cause of
this pathogenesis. Previously, we demonstrated that silica induced acute radical generation and
intracellular Ca2+ increase in Rat2 cells. Moreover, increases in intracellular Ca2+ directly
affect the rapid degradation of peroxiredoxin (Prx), a newly discovererd antioxidant enzyme
family. In the present study, we investigated the mechanism by which silica transfers its cellular
signal downstream of the cell by using human lung epithelial cells, which are known to
contribute to the inflammatory response after exposure to silica in the lung. In A549 cells, silica
induced the generation of ROS immediately, although immediate responses were observed in
C6 cells or in Rat2 cells. Nevertheless, the immediate production of ROS in response to silica
in A549 cells was not clearly detected, but the initial step for NF-κB signaling, such as rapid
degradation of IκB-α, was sensitive to the addition of silica. Additionally, silica triggered the
rapid degradation of cytoplasmic antioxidant enzymes such as PrxI and PrxII, indicating that
this may accelerate silica-induced cellular damage. Moreover, silica was observed to increase
intracellular Ca2+ concentration. This report suggests that silica transduces diverse pathways,
such as the rapid increase of intracellular Ca2+ concentration, NF-κB signaling or modulation
of redox potential by destruction of antioxidant enzymes, without the immediate generation of
ROS in A549 cells.