Cited 9 times in
Molecular detection of early appearance of drug resistance during Mycobacterium tuberculosis infection
DC Field | Value | Language |
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dc.contributor.author | 조상래 | - |
dc.date.accessioned | 2016-05-16T11:05:23Z | - |
dc.date.available | 2016-05-16T11:05:23Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 1434-6621 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143763 | - |
dc.description.abstract | During the early development of drug resistance in Mycobacterium tuberculosis (M. tuberculosis) infection only a small proportion of resistant bacteria are present within a milieu of sensitive bacteria. This complicates the use of molecular methods to predict the presence of a resistant phenotype and has been largely ignored in many of the newly developed molecular methods. In this study, mixtures of DNA from M. tuberculosis strains with known wild-type and mutant sequences were used to evaluate the sensitivity of three different molecular methods for detection of drug resistance. The dot-blot and amplification refractory mutation system (ARMS) methods showed sensitivities that approach those of routine phenotypic methods and are able to detect the presence of mutant sequences at a ratio of 1 in 50 (corresponding to 2% mutant sequences). This is 10-fold more sensitive than the commercial kit. The ARMS method was also used to investigate the use of molecular methods to identify mixed infections, and both drug-resistant and susceptible strain populations were identified in a single clinical isolate. These findings highlight the applicability of molecular methods to the rapid detection of drug resistance in tuberculosis patients, particularly in those who are non-compliant and in contacts of known drug-resistant tuberculosis patients, and assistance in limiting the spread of drug-resistant strains. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 876~881 | - |
dc.relation.isPartOf | CLINICAL CHEMISTRY AND LABORATORY MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | DNA Mutational Analysis/classification | - |
dc.subject.MESH | DNA Primers/genetics | - |
dc.subject.MESH | DNA Probes/genetics | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mycobacteriumtuberculosis/drugeffects | - |
dc.subject.MESH | Mycobacteriumtuberculosis/genetics* | - |
dc.subject.MESH | Mycobacteriumtuberculosis/isolation & purification | - |
dc.subject.MESH | Nucleic Acid Hybridization/methods | - |
dc.subject.MESH | Polymerase Chain Reaction/methods | - |
dc.subject.MESH | Reagent Kits, Diagnostic/microbiology | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | Tuberculosis, Multidrug-Resistant/microbiology* | - |
dc.title | Molecular detection of early appearance of drug resistance during Mycobacterium tuberculosis infection | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Thomas C. Victor | - |
dc.contributor.googleauthor | Hyeyoung Lee | - |
dc.contributor.googleauthor | Sang Nae Cho | - |
dc.contributor.googleauthor | Annemarie M. Jordaan | - |
dc.contributor.googleauthor | Gian van der Spuy | - |
dc.contributor.googleauthor | Paul D. van Helden | - |
dc.contributor.googleauthor | Robin Warren | - |
dc.identifier.doi | 10.1515/CCLM.2002.155 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03824 | - |
dc.relation.journalcode | J00567 | - |
dc.identifier.eissn | 1437-4331 | - |
dc.identifier.pmid | 12435103 | - |
dc.identifier.url | http://www.degruyter.com/view/j/cclm.2002.40.issue-9/cclm.2002.155/cclm.2002.155.xml | - |
dc.contributor.alternativeName | Cho, Sang Nae | - |
dc.contributor.affiliatedAuthor | Cho, Sang Nae | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 40 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 876 | - |
dc.citation.endPage | 881 | - |
dc.identifier.bibliographicCitation | CLINICAL CHEMISTRY AND LABORATORY MEDICINE, Vol.40(9) : 876-881, 2002 | - |
dc.identifier.rimsid | 37533 | - |
dc.type.rims | ART | - |
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