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추간판 세포에서 Transforming Growth Factor-β1 및 Bone Morphogenetic Protein-2에 의한 기질 생성 및 연골성 표현형 발현

Other Titles
 Transforming Growth Factor-beta1 and Bone Morphogenetic Protein-2 Upregulates Matrix Synthesis and Chondrogenic Phenotype in Intervertebral Disc Cells 
 김동준  ;  문성환  ;  김향  ;  권언혜  ;  한수봉  ;  천용민  ;  김학선  ;  한경진  ;  박문수  ;  이환모 
 Journal of Korean Society of Spine Surgery (대한척추외과학회지), Vol.9(3) : 165-171, 2002 
Journal Title
 Journal of Korean Society of Spine Surgery (대한척추외과학회지) 
Issue Date
TGF-β1 ; BMP-2 ; Aggrecan ; Collagen I ; Collagen II ; Osteocalcin
Objectives To determine effect of transforming growth factor-β1 and bone morphogenetic protein-2 in matrix synthesis and expression of chondrogenic phenotype in human intervertebral disc cells. Materials and Methods The intervertebral disc cells were harvested and cultured from the surgical patients for the degenerative disc disease. TGF-β1 was purchased from R&D and BMP-2 was produced by transfection of pcDNA3.1/Hygro/BMP-2 to CHO cell using Lipofectamine 2000. rhBMP-2 was separated by Heparin-Sepharose A chromatography. TGF-β1 a n d BMP-2 were administered to culture. Proteoglycan synthesis was assessed by 35S incorporation and expression of matrix mRNA was analyzed by RT-PCR for collagen I, collagen II, aggrecan, and osteocalcin. Results TGF-β1 and BMP-2 showed increased proteoglycan synthesis and expression of collagen I, collagen II and aggrecan mRNA in dose dependent manner respectively. There was no recognizable synergistic effect in matrix synthesis and matrix mRNA expression. Throughout dosage, expression of osteogenic phenotype (osteocalcin mRNA) was not noted. Conclusion TGF-β1 and BMP-2 proved to be effective anabolic agent for maximizing matrix synthesis without evidence of osteogenesis.
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1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hak Sun(김학선) ORCID logo https://orcid.org/0000-0002-8330-4688
Moon, Seong Hwan(문성환)
Park, Moon Soo(박문수)
Lee, Hwan Mo(이환모) ORCID logo https://orcid.org/0000-0002-5405-3832
Chun, Yong Min(천용민) ORCID logo https://orcid.org/0000-0002-8147-6136
Hahn, Soo Bong(한수봉)
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