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Quantitative measurement of Attachment forces between Cells and Polymeric Scaffolds

Other Titles
 세포와 고분자 표면간의 정량적 부착력 측정 
 Young Jick Kim  ;  Jung Woog Shin  ;  Ki Dong Park  ;  Jin Woo Lee  ;  Jeong Koo Kim  ;  Kyoung Soo Jee  ;  Gyu Ha Ryu 
 Biomaterials Research (생체재료학회지), Vol.6(2) : 65-71, 2002 
Journal Title
Biomaterials Research(생체재료학회지)
Issue Date
Quantitative measurement ; chondrocyle ; Polymeric Scaffold ; Attachment Force
Quantitative measurement of cell adhesion force to biomaterial surfaces was proposed and evaluated utilizing beam deflection theory with inverse finite element approach. The system for this measurement mainly consisted of a micropipette as a cantilever beam and digital image processing to measure the deflection amounts. Freshly isolated chondrocytes from mature porcine knee joints were seeded on three widely used biodegradable polymers: Poly -carprolactone (PCL), Poly(L-Lactide)(PLLA), Poly(Lactic-co-Glycolic acid, L:G=75:25) (PLGA). Detachment forces were measured at various time intervals, from 8 hours up to 5 days. The morphology was also observed at 12, and 24 hours after seeding. The cells seeded on the PLLA showed the highest attachment force among the three kinds of polymers at 8, 12, and 24 hours after seeding (p<0.05). No significant difference was found between PCL and PLGA at 12 hours after seeding. At 5 days after seeding, no significant difference was found between PCL, PLLA, and PLGA. This quantitative measurement technique may be potentially useful to analyze internal and external factors affecting the cell adhesion.
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1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
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