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UV-vulnerability of human papilloma virus type-16 E7-expressing astrocytes is associated with mitochondrial membrane depolarization and caspase-3 activation.
DC Field | Value | Language |
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dc.contributor.author | 박경아 | - |
dc.contributor.author | 이원택 | - |
dc.date.accessioned | 2016-05-16T11:02:22Z | - |
dc.date.available | 2016-05-16T11:02:22Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143651 | - |
dc.description.abstract | The human papilloma virus-type 16 (HPV-16) E6 and E7 proteins interact with the p53 and pRb tumor suppressor proteins, respectively. The effect of E6 or E7 expression on UV irradiation (5 and 20 J/m2)-induced genotoxic injury of confluent primary murine astrocytes was determined. Retroviral vectors were used to overexpress E6 and E7. Astrocytes expressing E7 showed increased vulnerability to UV-induced apoptosis while E6 over expressing astrocytes were protected from the same insults. Cell death in the E7 overexpressing cells was apoptotic because it showed DNA ladders, activation of caspase-3, formation of apoptotic bodies and decreased DNA content to less than the G0 level. After UV-irradiation the level of E2F1 in E7-expressing astrocytes was higher than E6-, LXSN- or mock-infected cells, and caspase-3 was activated to a greater extent. E7-expressing astrocytes showed the highest levels of Bax under normal growth conditions. The mitochondrial membrane potential of E7-expressing astrocytes was depolarized by 90% after UV-irradiation while the depolarization in control cells was about 50%. E6 overexpression decreased while E7 overexpression increased UV-induced astrocyte apoptosis. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 288~294 | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | UV-vulnerability of human papilloma virus type-16 E7-expressing astrocytes is associated with mitochondrial membrane depolarization and caspase-3 activation. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anatomy (해부학) | - |
dc.contributor.googleauthor | Won Taek Lee | - |
dc.contributor.googleauthor | Sung Ho Lee | - |
dc.contributor.googleauthor | Sean G. Carriedo | - |
dc.contributor.googleauthor | Rona G. Giffard | - |
dc.contributor.googleauthor | Yeon Jung Yoon | - |
dc.contributor.googleauthor | Ji Hyung Kim | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01424 | - |
dc.contributor.localId | A03007 | - |
dc.relation.journalcode | J02273 | - |
dc.identifier.eissn | 0219-1032 | - |
dc.subject.keyword | Apoptosis | - |
dc.subject.keyword | Astrocyte | - |
dc.subject.keyword | Bax | - |
dc.subject.keyword | Caspase-3 | - |
dc.subject.keyword | Human Papilloma Virus E6 and E7 Genes | - |
dc.subject.keyword | UV Irradiation | - |
dc.contributor.alternativeName | Park, Kyung Ah | - |
dc.contributor.alternativeName | Lee, Won Taek | - |
dc.contributor.affiliatedAuthor | Park, Kyung Ah | - |
dc.contributor.affiliatedAuthor | Lee, Won Taek | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 14 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 288 | - |
dc.citation.endPage | 294 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, Vol.14(2) : 288-294, 2002 | - |
dc.identifier.rimsid | 38328 | - |
dc.type.rims | ART | - |
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