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골 대체물 개발을 위한 열처리한 돼지 해면골의골형성에 대한 평가

Other Titles
 Evaluation of bone formation in the natural heat-treated porcine trabecular bone as bone substitute 
Authors
 박수아  ;  신정욱  ;  양영일  ;  조인희  ;  이진우  ;  박기동  ;  김영곤 
Citation
 Biomaterials Research (생체재료학회지), Vol.6(3) : 73-79, 2002 
Journal Title
Biomaterials Research(생체재료학회지)
ISSN
 1226-4601 
Issue Date
2002
Keywords
Heat-treated Porcine Bone Block ; Bone Marrow Stromal Cells(BmscS) ; Static Culture ; Dynamic culture
Abstract
The purpose of this study is to investigate the potential of tissue engineered heat-treated porcine trabecular bones as a bone substitute through three dimensional culture system with bone marrow stromal cells (BMSCs). Structural characteristics, mechanical stiffness and chemical composition were investigated. BMSCs were dynamically seeded into the block and cultured by either static or dynamic culturing condition. The cell-seeded bone blocks were examined histologically starting from 3 days up to 21 days after culture. The effects of seeding and culturing conditions on the tissue generation were examined. The effect of the collagen coating on the cell adhesion at initial stage was also investigated. The heat-treated porcine bone block was found to be hydroxyapatite (HA)-based material. It had high porosity (pore size: 300-500 ?m) and had similar strength to the human trabecular bones (Young’s modulus=346.33 MPa). Observation by scanning electron microscopy (SEM), H&E staining and transmission electron microscopy (TEM) revealed bony tissue regeneration such as the cell adhesion, the formation of osteoids, and generation of lamellar bone and woven bone. The result showed that the dynamic culture was superior to static culture. Although there was no significant effect of the collagen coating on the cell activities, the attachment was improved in collagen coated bone blocks. Based on all the results obtained from this study, we could conclude this heat-treated porcine trabecular bone had the potential of the xenograft for bone regeneration.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143559
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