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RPMI 배지 및 RPMI-2% glucose 배지를 이용한 Candida albicans의 fluconazole 감수성 검사

Other Titles
 Evaluation of a Spectrophotometric Broth Microdilution Method for Determining Fluconazole Susceptibility of Candida albicans: Influence of RPMI and RPMI-2% Glucose on the Selection of Endpoint Criteria 
Authors
 이지연  ;  신종희  ;  이경원  ;  용동은  ;  양성진  ;  서순팔  ;  양동욱 
Citation
 KOREAN JOURNAL OF LABORATORY MEDICINE , Vol.22(3) : 188-195, 2002 
Journal Title
 KOREAN JOURNAL OF LABORATORY MEDICINE 
ISSN
 1598-6535 
Issue Date
2002
Keywords
Spectrophotometric broth microdilution method ; Fluconazole ; Candida albicans ; RPMI-2% glucose
Abstract
Background :Minimum inhibitory concentration (MIC) endpoint determination is the major variation source for the fluconazole susceptibility test, especially for Candida albicans. In this study, we evaluated spectrophotometric broth microdilution methods using RPMI 1640 and RPMI supplemented with 18 g of glucose per liter (RPMI-2% glucose) for determining fluconazole susceptibility of C. albicans. Method :A total of 129 clinical isolates of C. albicans were tested by the broth microdilution method using RPMI and RPMI-2% glucose. The MIC endpoint was calculated objectively with the spectrophotometer set at 405 nm. These results were compared to those by the National Committee for Clinical Laboratory Standards (NCCLS) macrodilution method and the agar dilution method. Result :The mean absorbances in the drug-free wells in RPMI and RPMI-2% glucose were 0.208±0.014 and 0.316±0.061, respectively, at 24 h and 0.339±0.094 and 0.530±0.104, respectively, at 48 h (P<0.01). Conclusion :The spectrophotometric microdilution method with RPMI-2% glucose may have an excellent agreement with the NCCLS broth macrodilution method and may provide more easily determined MIC endpoints for fluconazole susceptibility testing for C. albicans.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143517
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