Cited 5 times in
Oxidant-sensitive transcription factor and cyclooxygenase-2 by Helicobacter pylori stimulation in human gastric cancer cells
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.contributor.author | 임주원 | - |
dc.date.accessioned | 2016-05-16T10:55:04Z | - |
dc.date.available | 2016-05-16T10:55:04Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0731-8898 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143397 | - |
dc.description.abstract | Helicobacter pylori (H. pylori) infection might activate nuclear factor-kB (NF-kB), an oxidant-sensitive transcription regulator of inducible expression of inflammatory genes such as cyclooxygenase-2 (COX-2). We studied the role of NF-kB on expression of COX-2 in H. pylori-stimulated gastric cancer cell lines by using antioxidants, glutathione (GSH), and N-acetylcysteine (NAC) as well as an NF-kB inhibitor, pyrrolidine dithiocarbamate (PDTC). Gastric adenocarcinoma cell lines derived from Caucasian (AGS) cells and Korean (SNU-484) cells were used to study the role of NF-kB on COX-2 expression by H. pylori. They were treated with GSH, NAC, or PDTC in the presence of H. pylori. mRNA expression and protein level for COX-2 were determined by Northern blot and RT-PCR analysis as well as Western blot analysis. NF-kB activation was examined by electrophoretic mobility shift assay. As a result, H. pylori induced a time-dependent expression of mRNA and protein for COX-2 via activation of NF-kB, which was inhibited by GSH, NAC, and PDTC in the cells. In conclusion, oxidant-sensitive transcription factor NF-kB may play a novel role in expression of COX-2 by H. pylori stimulation in gastric cancer cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 121~129 | - |
dc.relation.isPartOf | JOURNAL OF ENVIRONMENTAL PATHOLOGY TOXICOLOGY AND ONCOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Oxidant-sensitive transcription factor and cyclooxygenase-2 by Helicobacter pylori stimulation in human gastric cancer cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Hyeyoung Kim | - |
dc.contributor.googleauthor | Joo Weon Lim | - |
dc.contributor.googleauthor | Jeong Yeon Seo | - |
dc.contributor.googleauthor | Kyung Hwan Kim | - |
dc.identifier.doi | 10.1615/JEnvironPatholToxicolOncol.v21.i2.50 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03405 | - |
dc.contributor.localId | A00311 | - |
dc.relation.journalcode | J01400 | - |
dc.identifier.eissn | 2162-6537 | - |
dc.identifier.url | http://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,4ac6c8531cba5202,419b49a4498fdc48.html | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.alternativeName | Lim, Joo Weon | - |
dc.contributor.affiliatedAuthor | Lim, Joo Weon | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hwan | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 21 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 121 | - |
dc.citation.endPage | 129 | - |
dc.identifier.bibliographicCitation | JOURNAL OF ENVIRONMENTAL PATHOLOGY TOXICOLOGY AND ONCOLOGY, Vol.21(2) : 121-129, 2002 | - |
dc.identifier.rimsid | 53138 | - |
dc.type.rims | ART | - |
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