Cited 25 times in
Transcriptional regulation by thiol compounds in Helicobacter pylori-induced IL-8 production in human gastric epithelial cells
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.date.accessioned | 2016-05-16T10:54:48Z | - |
dc.date.available | 2016-05-16T10:54:48Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0077-8923 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143389 | - |
dc.description.abstract | Reactive oxygen species (ROS) have been counted among the potential toxic factors involving Helicobacter pylori (H. pylori)-induced gastric injury. Transcription nuclear factor-κB (NF-κB) is activated by ROS and regulates inflammatory gene expression. Thiol compounds, such as glutathione and N-acetylcysteine, scavenge hydrogen peroxide and are reported to prevent oxidative damage in various cells. The present study aims to investigate whether thiol compounds could affect H. pylori-induced IL-8 production by regulating transcription factor NF-κB in human gastric epithelial AGS cells. AGS cells were incubated with H. pylori (NCTC 11637) at a ratio of 1:100 in the presence or absence of thiol compounds. ROS generation was determined by confocal microscopy using ROS-sensitive dichlorofluorescein diacetate dye. Levels of hydrogen peroxide and IL-8 in the medium and DNA binding activity of NF-κB were determined by enzyme-linked immunosorbent assay, colorimetric assay, and electrophoretic mobility shift assay. Results indicated both thiol compounds inhibited H. pylori-induced hydrogen peroxide production, in accordance with their inhibition on NF-κB activation and IL-8 production induced by H. pylori in AGS cells. In conclusion, ROS may be a signaling molecule triggering NF-κB activation and the expression of inflammatory genes such as IL-8. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 541~545 | - |
dc.relation.isPartOf | ANNALS OF THE NEW YORK ACADEMY OF SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Transcriptional regulation by thiol compounds in Helicobacter pylori-induced IL-8 production in human gastric epithelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Jeong Yeon Seo | - |
dc.contributor.googleauthor | Hyeyoung Kim | - |
dc.contributor.googleauthor | Kyung Hwan Kim | - |
dc.identifier.doi | 10.1111/j.1749-6632.2002.tb04697.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00311 | - |
dc.relation.journalcode | J00181 | - |
dc.identifier.eissn | 1749-6632 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1749-6632.2002.tb04697.x/abstract | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hwan | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 973 | - |
dc.citation.startPage | 541 | - |
dc.citation.endPage | 545 | - |
dc.identifier.bibliographicCitation | ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol.973 : 541-545, 2002 | - |
dc.identifier.rimsid | 53131 | - |
dc.type.rims | ART | - |
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