Cited 18 times in
Effect of mannitol on helicobacter pylori-induced cyclooxygenase-2 expression in gastric epithelial AGS cells.
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.date.accessioned | 2016-05-16T10:54:47Z | - |
dc.date.available | 2016-05-16T10:54:47Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0031-7012 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143388 | - |
dc.description.abstract | Oxygen radicals have been considered to be important regulatory molecules in Helicobacter pylori-induced gastric ulceration and carcinogenesis. H. pylori-induced inflammation has been shown to be associated with cyclooxygenase-2 (COX-2) expression in experimental animals and human patients. This study aimed to determine if H. pylori produces oxygen radicals and induces COX-2 expression in gastric epithelial cells. A further aim was to resolve whether or not the H. pylori-induced COX-2 expression could be inhibited by mannitol, a known hydroxyl radical scavenger, and superoxide dismutase (SOD), an antioxidant enzyme, which was used as a positive control. A human gastric epithelial cell line, AGS, treated with or without mannitol or SOD, was incubated in the presence or absence of H. pylori. mRNA expression and protein levels for COX-2 were determined by Northern blot and Western blot analysis, respectively. Levels of the COX-2 products, 6-keto-prostaglandin F1α (6-keto-PGF1α) and thromboxane B2 (TXB2) and H2O2 were measured in the medium. The results showed that H. pylori induced H2O2 production, COX-2 mRNA and protein expression and increased the levels of 6-keto-PGF1α and TXB2. The H. pylori-induced COX-2 expression and the increase in the COX-2 products were inhibited by both mannitol and SOD. The inhibitory effect of mannitol on H. pylori-induced COX-2 expression suggests the possible involvement of oxygen radicals in the transcriptional regulation of the inflammatory mediators in gastric epithelial cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 182~189 | - |
dc.relation.isPartOf | PHARMACOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Effect of mannitol on helicobacter pylori-induced cyclooxygenase-2 expression in gastric epithelial AGS cells. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Hyeyoung Kim | - |
dc.contributor.googleauthor | Jeong Yeon Seo | - |
dc.contributor.googleauthor | Kyung Hwan Kim | - |
dc.identifier.doi | 10.1159/000065532 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00311 | - |
dc.relation.journalcode | J02509 | - |
dc.identifier.eissn | 1423-0313 | - |
dc.identifier.url | http://www.karger.com/Article/FullText/65532 | - |
dc.subject.keyword | Mannitol | - |
dc.subject.keyword | Cyclooxygenase-2 | - |
dc.subject.keyword | Helicobacter pylori | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hwan | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 66 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 182 | - |
dc.citation.endPage | 189 | - |
dc.identifier.bibliographicCitation | PHARMACOLOGY, Vol.66(4) : 182-189, 2002 | - |
dc.identifier.rimsid | 53130 | - |
dc.type.rims | ART | - |
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