Cited 23 times in
Glucocorticoid inhibits growth factor-induced differentiation of hippocampal progenitor HiB5 cells
DC Field | Value | Language |
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dc.contributor.author | 정호성 | - |
dc.date.accessioned | 2016-02-19T11:28:45Z | - |
dc.date.available | 2016-02-19T11:28:45Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0022-3042 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143230 | - |
dc.description.abstract | In the present study, we investigated the effect of glucocorticoid on neuronal differentiation of hippocampal progenitor HiB5 cells. Dexamethasone (DEX), a synthetic glucocorticoid, inhibited platelet-derived growth factor (PDGF)-induced differentiation of HiB5 cells. The inhibitory effect of DEX was antagonized by RU486, a glucocorticoid receptor (GR) antagonist, indicating the GR-mediated processes. Nestin mRNA level was decreased and midsize neurofilament (NF-M) mRNA level was increased as a function of neuronal differentiation. DEX significantly blocked PDGF-induced down-regulation of nestin mRNA level, and up-regulation of NF-M mRNA level, which were similar to those of undifferentiated cells. DEX inhibited PDGF-induced activation of cyclic AMP-responsive element binding protein (CREB) and AP-1, suggesting that glucocorticoid interfered with signal transduction cascades linking the PDGF receptor and downstream transcription factors. Indeed, DEX reduced PDGF-induced phosphorylation of extracellular signal-regulated kinases1/2 (ERK1/2). Tyrosine phosphatase inhibitor reversed the effect of DEX on ERK1/2. In accordance with this finding, blockage of ERK1/2 signaling pathway with PD098059, a potent inhibitor for Ras/ERK pathway, mimicked the inhibitory effect of DEX on differentiation processes. Taken together, these results indicate that glucocorticoid inhibits PDGF-induced differentiation of hippocampal progenitor HiB5 cells by inhibiting the ERK1/2 signaling cascade via a tyrosine phosphatase-dependent mechanism. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1013~1021 | - |
dc.relation.isPartOf | JOURNAL OF NEUROCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Blotting, Southern | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cell Differentiation/drug effects | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Dexamethasone/pharmacology | - |
dc.subject.MESH | Glucocorticoids/pharmacology* | - |
dc.subject.MESH | Growth Substances/pharmacology* | - |
dc.subject.MESH | Hippocampus/cytology* | - |
dc.subject.MESH | Hippocampus/drug effects | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Luciferases/metabolism | - |
dc.subject.MESH | Neurites/drug effects | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Platelet-Derived Growth Factor/metabolism | - |
dc.subject.MESH | Protein Tyrosine Phosphatases/metabolism | - |
dc.subject.MESH | Receptors, Glucocorticoid/biosynthesis | - |
dc.subject.MESH | Receptors, Glucocorticoid/drug effects | - |
dc.subject.MESH | Receptors, Mineralocorticoid/biosynthesis | - |
dc.subject.MESH | Receptors, Mineralocorticoid/drug effects | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Signal Transduction/drug effects | - |
dc.subject.MESH | Stem Cells/drug effects* | - |
dc.subject.MESH | Transfection | - |
dc.title | Glucocorticoid inhibits growth factor-induced differentiation of hippocampal progenitor HiB5 cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anatomy (해부학) | - |
dc.contributor.googleauthor | Gi Hoon Son | - |
dc.contributor.googleauthor | Dongho Geum | - |
dc.contributor.googleauthor | Hosung Jung | - |
dc.contributor.googleauthor | Kyungjin Kim | - |
dc.identifier.doi | 10.1046/j.1471-4159.2001.00634.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03786 | - |
dc.relation.journalcode | J01620 | - |
dc.identifier.eissn | 1471-4159 | - |
dc.identifier.pmid | 11739613 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1046/j.1471-4159.2001.00634.x/abstract | - |
dc.subject.keyword | differentiation | - |
dc.subject.keyword | extracellular signal‐regulated kinases1/2 | - |
dc.subject.keyword | glucocorticoid | - |
dc.subject.keyword | hippocampal progenitor | - |
dc.subject.keyword | protein tyrosine phosphatase | - |
dc.contributor.alternativeName | Jung, Ho Sung | - |
dc.contributor.affiliatedAuthor | Jung, Ho Sung | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 79 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1013 | - |
dc.citation.endPage | 1021 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NEUROCHEMISTRY, Vol.79(5) : 1013-1021, 2001 | - |
dc.identifier.rimsid | 39154 | - |
dc.type.rims | ART | - |
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