Cited 41 times in

Expression of hMLH1 is inactivated in the gastric adenomas with enhanced microsatellite instability

Authors
 M J Baek  ;  H Kang  ;  S E Kim  ;  J H Park  ;  J S Lee  ;  Y-K Paik  ;  H Kim 
Citation
 BRITISH JOURNAL OF CANCER, Vol.85(8) : 1147-1152, 2001 
Journal Title
BRITISH JOURNAL OF CANCER
ISSN
 0007-0920 
Issue Date
2001
MeSH
Adaptor Proteins, Signal Transducing ; Adenoma/genetics* ; Carrier Proteins ; DNA Methylation ; DNA-Binding Proteins* ; Frameshift Mutation ; Humans ; Microsatellite Repeats* ; MutL Protein Homolog 1 ; MutS Homolog 2 Protein ; Neoplasm Proteins/genetics* ; Nuclear Proteins ; Promoter Regions, Genetic ; Proto-Oncogene Proteins/genetics ; Stomach Neoplasms/genetics*
Keywords
gastric adenoma ; gastric carcinoma ; microsatellite instability ; hMLH1 ; hMSH2
Abstract
Microsatellite instability (MSI) and frameshift mutations in the genes containing coding nucleotide repeats have been reported in a subset of gastric adenomas, however the inactivation profiles of DNA mismatch repair genes in MSI-positive gastric adenomas have not been characterized. To address the origin of MSI in gastric adenomas, expressions of hMLH1 and hMSH2 were explored in 86 gastric adenomas. Gastric carcinomas, of which 16 were MSI-positive and 22 MSI-negative, were used as controls. MSI was found in 15 (17%) of gastric adenomas. Absent or decreased hMLH1 expression by immunohistochemistry was noted in most of the MSI-positive adenomas (13/15, 87%) and carcinomas (14/16, 88%), and all of these tumours showed methylation of the hMLH1 gene promoter. In contrast, rare inactivation of hMLH1 expression was found in MSI-negative adenomas (3/71, 4%) and carcinomas (2/22, 9%). Intense expression of hMSH2 gene product was observed in most of the gastric adenomas and carcinomas regardless of MSI status. These findings indicate that the inactivation of hMLH1 gene expression by promoter methylation is an early event and might be the origin of MSI-positive gastric adenomas.
Files in This Item:
T200103912.pdf Download
DOI
10.1054/bjoc.2001.2051
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Hyun Ju(강현주)
Kim, Hogeun(김호근)
Park, Jeon Han(박전한) ORCID logo https://orcid.org/0000-0001-9604-3205
Lee, Joon Soo(이준수) ORCID logo https://orcid.org/0000-0001-9036-9343
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143211
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links