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Proteome analysis of metabolically engineered Escherichia coli producing poly(3-hydroxybutyrate)

DC Field Value Language
dc.contributor.author윤상선-
dc.date.accessioned2016-02-19T11:28:00Z-
dc.date.available2016-02-19T11:28:00Z-
dc.date.issued2001-
dc.identifier.issn0021-9193-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/143205-
dc.description.abstractRecombinant Escherichia coli strains harboring heterologous polyhydroxyalkanoate (PHA) biosynthesis genes were shown to accumulate unusually large amounts of PHA. In the present study, integrated cellular responses of metabolically engineered E. coli to the accumulation of poly(3-hydroxybutyrate) (PHB) in the early stationary phase were analyzed at the protein level by two-dimensional gel electrophoresis. Out of 20 proteins showing altered expression levels with the accumulation of PHB, 13 proteins were identified with the aid of mass spectrometry. Three heat shock proteins, GroEL, GroES, and DnaK, were significantly up-regulated in PHB-accumulating cells. Proteins which play essential roles in protein biosynthesis were unfavorably influenced by the accumulation of PHB. Cellular demand for the large amount of acetyl coenzyme A and NADPH for the PHB biosynthesis resulted in the increased synthesis of two enzymes of the glycolytic pathway and one enzyme of the Entner-Doudoroff pathway. The expression of the yfiD gene encoding a 14.3-kDa protein, which is known to be produced at low pH, was greatly induced with the accumulation of PHB. Therefore, it could be concluded that the accumulation of PHB in E. coli acted as a stress on the cells, which reduced the cells' ability to synthesize proteins and induced the expression of various protective proteins.-
dc.description.statementOfResponsibilityopen-
dc.format.extent301~308-
dc.relation.isPartOfJOURNAL OF BACTERIOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBacterial Proteins/metabolism-
dc.subject.MESHElectrophoresis, Gel, Two-Dimensional/methods-
dc.subject.MESHEscherichia coli/genetics*-
dc.subject.MESHEscherichia coli/growth & development-
dc.subject.MESHEscherichia coli/metabolism*-
dc.subject.MESHGenetic Engineering*-
dc.subject.MESHHydroxybutyrates/metabolism*-
dc.subject.MESHProteome*/metabolism*-
dc.subject.MESHRecombination, Genetic-
dc.subject.MESHSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods-
dc.titleProteome analysis of metabolically engineered Escherichia coli producing poly(3-hydroxybutyrate)-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Microbiology (미생물학)-
dc.contributor.googleauthorMee-Jung Han-
dc.contributor.googleauthorSang Sun Yoon-
dc.contributor.googleauthorSang Yup Lee-
dc.identifier.doi10.1128/JB.183.1.301-308.2001-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02558-
dc.relation.journalcodeJ01254-
dc.identifier.eissn1098-5530-
dc.identifier.pmid11114930-
dc.contributor.alternativeNameYoon, Sang Sun-
dc.contributor.affiliatedAuthorYoon, Sang Sun-
dc.rights.accessRightsfree-
dc.citation.volume183-
dc.citation.number1-
dc.citation.startPage301-
dc.citation.endPage308-
dc.identifier.bibliographicCitationJOURNAL OF BACTERIOLOGY, Vol.183(1) : 301-308, 2001-
dc.identifier.rimsid39139-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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