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A Bone Replaceable Artificial Bone Substitute: Osteoinduction by Combining with Bone Inducing Agent

DC FieldValueLanguage
dc.contributor.author박종철-
dc.contributor.author서활-
dc.contributor.author한창동-
dc.date.accessioned2016-02-19T11:26:59Z-
dc.date.available2016-02-19T11:26:59Z-
dc.date.issued2001-
dc.identifier.issn0160-564X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/143168-
dc.description.abstractBone inducing agent (BIA) isolated from Saos-2 human osteosarcoma cells was added to an artificial bone substitute composed of 980°C-heated carbonate apatite (CAp) and Type I atelocollagen (AtCol) extracted from bovine tail skins (88/12 in wt/wt %), and a CAp-AtCol-BIA substitute was prepared as an osteoinductive bone substitute. Rat calvaria osteoblasts treated by the isolated BIA demonstrated significantly increased alkaline phosphatase (ALP) activity after 3 days (p < 0.05). In vitro cell attachment and proliferation and ALP activity were investigated for the bone substitute combined with BIA. Osteoblasts cultured onto the surface of the CAp-AtCol-BIA substitute demonstrated remarkable morphological changes such as radial spreading, flattening, and projecting filopodia after 5 days. In comparison with the substitute without BIA, osteoblasts grown in the BIA-combined CAp-AtCol substitute expressed significantly increased proliferation and ALP activity, respectively (p < 0.05). Both the substitutes combined with and without BIA were implanted into artificial defects created in rabbit radii. After 4 weeks, the CAp-AtCol-BIA substitute implanted lesion was completely replaced by regenerated host bone in radiological observation whereas the substitute without BIA was partially resorbed. No histologic abnormalities appeared in the substitute either with or without BIA.-
dc.description.statementOfResponsibilityopen-
dc.format.extent459~466-
dc.relation.isPartOfARTIFICIAL ORGANS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAlkaline Phosphatase/metabolism-
dc.subject.MESHAnalysis of Variance-
dc.subject.MESHAnimals-
dc.subject.MESHBiological Factors/chemistry-
dc.subject.MESHBiological Factors/pharmacology*-
dc.subject.MESHBone Neoplasms/metabolism-
dc.subject.MESHBone Substitutes/chemistry-
dc.subject.MESHBone Substitutes/pharmacology*-
dc.subject.MESHCattle-
dc.subject.MESHCell Adhesion/drug effects-
dc.subject.MESHCollagen/chemistry-
dc.subject.MESHCollagen/pharmacology*-
dc.subject.MESHOsteoblasts/cytology-
dc.subject.MESHOsteoblasts/drug effects*-
dc.subject.MESHOsteoblasts/enzymology-
dc.subject.MESHOsteosarcoma/metabolism-
dc.subject.MESHRabbits-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHTumor Cells, Cultured/metabolism-
dc.titleA Bone Replaceable Artificial Bone Substitute: Osteoinduction by Combining with Bone Inducing Agent-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Medical Engineering (의학공학)-
dc.contributor.googleauthorHwal Suh-
dc.contributor.googleauthorDong-Wook Han-
dc.contributor.googleauthorJong-Chul Park-
dc.contributor.googleauthorDong Hee Lee-
dc.contributor.googleauthorWoo Suk Lee-
dc.contributor.googleauthorChang Dong Han-
dc.identifier.doi10.1046/j.1525-1594.2001.025006459.x-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01662-
dc.contributor.localIdA01924-
dc.contributor.localIdA04330-
dc.relation.journalcodeJ00246-
dc.identifier.eissn1525-1594-
dc.identifier.pmid11453876-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1046/j.1525-1594.2001.025006459.x/abstract-
dc.subject.keywordCarbonate apatite-
dc.subject.keywordType I atelocollagen-
dc.subject.keywordBone inducing agent-
dc.subject.keywordOsteoblast attachment-
dc.subject.keywordOsteoblast proliferation-
dc.subject.keywordAlkaline phosphatase activity-
dc.contributor.alternativeNamePark, Jong Chul-
dc.contributor.alternativeNameSuh, Hwal-
dc.contributor.alternativeNameHan, Chang Dong-
dc.contributor.affiliatedAuthorPark, Jong Chul-
dc.contributor.affiliatedAuthorSuh, Hwal-
dc.contributor.affiliatedAuthorHan, Chang Dong-
dc.rights.accessRightsnot free-
dc.citation.volume25-
dc.citation.number6-
dc.citation.startPage459-
dc.citation.endPage466-
dc.identifier.bibliographicCitationARTIFICIAL ORGANS, Vol.25(6) : 459-466, 2001-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers

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