Cited 39 times in
Effects of Nonsteroidal Anti-Inflammatory Drugs on Helicobacter pylori-Infected Gastric Mucosae of Mice: Apoptosis, Cell Proliferation, and Inflammatory Activity
DC Field | Value | Language |
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dc.contributor.author | 강진경 | - |
dc.contributor.author | 김태일 | - |
dc.contributor.author | 박인서 | - |
dc.contributor.author | 이광형 | - |
dc.contributor.author | 이용찬 | - |
dc.contributor.author | 전재윤 | - |
dc.contributor.author | 한재호 | - |
dc.date.accessioned | 2016-02-19T11:26:24Z | - |
dc.date.available | 2016-02-19T11:26:24Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0019-9567 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143146 | - |
dc.description.abstract | Helicobacter pylori and nonsteroidal anti-inflammatory drugs (NSAIDs) are two well-known important causative factors of gastric damage. While H. pylori increases apoptosis and the proliferation of gastric epithelial cells and is an important factor in peptic ulcer and gastric cancer, NSAIDs induce cell apoptosis and have antineoplastic effects. We investigated the effects of NSAIDs (a nonselective cyclooxygenase [COX] inhibitor [indomethacin] and a selective COX-2 inhibitor [NS-398]) on the apoptosis and proliferation of gastric epithelial cells and gastric inflammation in H. pylori-infected mice. C57BL/6 mice were sacrificed 8 weeks after H. pylori SS1 inoculation. Indomethacin (2 mg/kg) or NS-398 (10 mg/kg) was administered subcutaneously once daily for 10 days before sacrifice. The following were assessed: gastric inflammatory activity, gastric COX protein expression by Western blotting; gastric prostaglandin E2 levels by enzyme immunoassay, apoptosis by terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling, and cell proliferation by Ki67 immunostaining. Compared to the controls, H. pylori infection and/or NSAID treatment increased COX-1 and COX-2 protein expression. Gastric prostaglandin E2 levels, apoptotic index, cell proliferation index, neutrophil activity, and the degree of chronic inflammation were all increased by H. pylori infection, and these effects were significantly decreased by indomethacin treatment. However, NS-398 treatment after H. pylori infection did not induce a significant reduction, although it did result in a tendency to decrease. These results show that NSAIDs can reverse the increased apoptosis and proliferation of epithelial cells and inflammatory activity in the stomachs of H. pylori-infected mice and that, like COX-2 activation, COX-1 induction contributes to the change of gastric mucosal cell turnover and inflammation induced by H. pylori infection. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 5056~5063 | - |
dc.relation.isPartOf | INFECTION AND IMMUNITY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Anti-Inflammatory Agents, Non-Steroidal/pharmacology* | - |
dc.subject.MESH | Apoptosis/drug effects* | - |
dc.subject.MESH | Apoptosis/immunology | - |
dc.subject.MESH | Cell Division | - |
dc.subject.MESH | Chronic Disease | - |
dc.subject.MESH | Cyclooxygenase 1 | - |
dc.subject.MESH | Cyclooxygenase 2 | - |
dc.subject.MESH | Cyclooxygenase 2 Inhibitors | - |
dc.subject.MESH | Cyclooxygenase Inhibitors/pharmacology | - |
dc.subject.MESH | Dinoprostone/metabolism | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Epithelial Cells/cytology | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gastric Mucosa/cytology | - |
dc.subject.MESH | Gastric Mucosa/drug effects* | - |
dc.subject.MESH | Gastric Mucosa/immunology | - |
dc.subject.MESH | Gastritis/immunology* | - |
dc.subject.MESH | Gastritis/pathology | - |
dc.subject.MESH | Helicobacter Infections/immunology* | - |
dc.subject.MESH | Helicobacter Infections/pathology | - |
dc.subject.MESH | Helicobacter pylori/immunology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Indomethacin/pharmacology* | - |
dc.subject.MESH | Isoenzymes/antagonists & inhibitors | - |
dc.subject.MESH | Isoenzymes/biosynthesis | - |
dc.subject.MESH | Membrane Proteins | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Nitrobenzenes/pharmacology* | - |
dc.subject.MESH | Prostaglandin-Endoperoxide Synthases/biosynthesis | - |
dc.subject.MESH | Sulfonamides/pharmacology* | - |
dc.title | Effects of Nonsteroidal Anti-Inflammatory Drugs on Helicobacter pylori-Infected Gastric Mucosae of Mice: Apoptosis, Cell Proliferation, and Inflammatory Activity | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Tae Il Kim | - |
dc.contributor.googleauthor | Yong Chan Lee | - |
dc.contributor.googleauthor | Kwang Hyoung Lee | - |
dc.contributor.googleauthor | Jae Ho Han | - |
dc.contributor.googleauthor | Chae Yoon Chon | - |
dc.contributor.googleauthor | Young Myoung Moon | - |
dc.contributor.googleauthor | Jin Kyung Kang | - |
dc.contributor.googleauthor | In Suh Park | - |
dc.identifier.doi | 10.1128/IAI.69.8.5056-5063.2001 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00085 | - |
dc.contributor.localId | A01079 | - |
dc.contributor.localId | A01626 | - |
dc.contributor.localId | A02673 | - |
dc.contributor.localId | A02988 | - |
dc.contributor.localId | A04321 | - |
dc.contributor.localId | A03544 | - |
dc.relation.journalcode | J01055 | - |
dc.identifier.eissn | 1098-5522 | - |
dc.identifier.pmid | 11447186 | - |
dc.contributor.alternativeName | Kang, Jin Kyung | - |
dc.contributor.alternativeName | Kim, Tae Il | - |
dc.contributor.alternativeName | Park, In Suh | - |
dc.contributor.alternativeName | Lee, Kwang Hyoung | - |
dc.contributor.alternativeName | Lee, Yong Chan | - |
dc.contributor.alternativeName | Chon, Chae Yoon | - |
dc.contributor.alternativeName | Han, Jae Ho | - |
dc.contributor.affiliatedAuthor | Kang, Jin Kyung | - |
dc.contributor.affiliatedAuthor | Kim, Tae Il | - |
dc.contributor.affiliatedAuthor | Park, In Suh | - |
dc.contributor.affiliatedAuthor | Lee, Kwang Hyoung | - |
dc.contributor.affiliatedAuthor | Lee, Yong Chan | - |
dc.contributor.affiliatedAuthor | Han, Jae Ho | - |
dc.contributor.affiliatedAuthor | Chon, Chae Yoon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 69 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 5056 | - |
dc.citation.endPage | 5063 | - |
dc.identifier.bibliographicCitation | INFECTION AND IMMUNITY, Vol.69(8) : 5056-5063, 2001 | - |
dc.identifier.rimsid | 39087 | - |
dc.type.rims | ART | - |
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