743 922

Cited 834 times in

Predominant role of endothelial nitric oxide synthase in VEGF-induced angiogenesis and vascular permeability

DC Field Value Language
dc.contributor.author윤채옥-
dc.date.accessioned2016-02-19T11:23:47Z-
dc.date.available2016-02-19T11:23:47Z-
dc.date.issued2001-
dc.identifier.issn0027-8424-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/143052-
dc.description.abstractNitric oxide (NO) plays a critical role in vascular endothelial growth factor (VEGF)-induced angiogenesis and vascular hyperpermeability. However, the relative contribution of different NO synthase (NOS) isoforms to these processes is not known. Here, we evaluated the relative contributions of endothelial and inducible NOS (eNOS and iNOS, respectively) to angiogenesis and permeability of VEGF-induced angiogenic vessels. The contribution of eNOS was assessed by using an eNOS-deficient mouse, and iNOS contribution was assessed by using a selective inhibitor [l-N6-(1-iminoethyl) lysine, l-NIL] and an iNOS-deficient mouse. Angiogenesis was induced by VEGF in type I collagen gels placed in the mouse cranial window. Angiogenesis, vessel diameter, blood flow rate, and vascular permeability were proportional to NO levels measured with microelectrodes: Wild-type (WT) ≥ WT with l-NIL or iNOS−/− > eNOS−/− ≥ eNOS−/− with l-NIL. The role of NOS in VEGF-induced acute vascular permeability increase in quiescent vessels also was determined by using eNOS- and iNOS-deficient mice. VEGF superfusion significantly increased permeability in both WT and iNOS−/− mice but not in eNOS−/− mice. These findings suggest that eNOS plays a predominant role in VEGF-induced angiogenesis and vascular permeability. Thus, selective modulation of eNOS activity is a promising strategy for altering angiogenesis and vascular permeability in vivo.-
dc.description.statementOfResponsibilityopen-
dc.format.extent2604~2609-
dc.relation.isPartOfPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBlood Circulation/physiology-
dc.subject.MESHCapillary Permeability/physiology*-
dc.subject.MESHEndothelial Growth Factors/physiology*-
dc.subject.MESHFemale-
dc.subject.MESHHomeodomain Proteins/genetics-
dc.subject.MESHHomeodomain Proteins/physiology-
dc.subject.MESHLymphokines/physiology*-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHMice, Knockout-
dc.subject.MESHNeovascularization, Physiologic/physiology*-
dc.subject.MESHNitric Oxide/metabolism-
dc.subject.MESHNitric Oxide Synthase/genetics-
dc.subject.MESHNitric Oxide Synthase/physiology*-
dc.subject.MESHNitric Oxide Synthase Type II-
dc.subject.MESHNitric Oxide Synthase Type III-
dc.subject.MESHVascular Endothelial Growth Factor A-
dc.subject.MESHVascular Endothelial Growth Factors-
dc.titlePredominant role of endothelial nitric oxide synthase in VEGF-induced angiogenesis and vascular permeability-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.departmentInstitute for Cancer Research (암연구소)-
dc.contributor.googleauthorDai Fukumura-
dc.contributor.googleauthorTakeshi Gohongi-
dc.contributor.googleauthorAnanth Kadambi-
dc.contributor.googleauthorYotaro Izumi-
dc.contributor.googleauthorJennifer Ang-
dc.contributor.googleauthorChae-Ok Yun-
dc.contributor.googleauthorDonald G. Buerk-
dc.contributor.googleauthorPaul L. Huang-
dc.contributor.googleauthorRakesh K. Jain-
dc.identifier.doi10.1073/pnas.041359198-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02614-
dc.relation.journalcodeJ02550-
dc.identifier.eissn1091-6490-
dc.identifier.pmid11226286-
dc.contributor.alternativeNameYun, Chae Ok-
dc.contributor.affiliatedAuthorYun, Chae Ok-
dc.rights.accessRightsfree-
dc.citation.volume98-
dc.citation.number5-
dc.citation.startPage2604-
dc.citation.endPage2609-
dc.identifier.bibliographicCitationPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol.98(5) : 2604-2609, 2001-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.