Cited 17 times in
TGF-βs Synthesized by RPE Cells Have Autocrine Activity on Mesenchymal Transformation and Cell Proliferation
DC Field | Value | Language |
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dc.contributor.author | 고형준 | - |
dc.date.accessioned | 2016-02-19T11:20:34Z | - |
dc.date.available | 2016-02-19T11:20:34Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142934 | - |
dc.description.abstract | The present study investigated the effects of transforming growth factor (TGF)-β on retinal pigment epithelial (RPE) transformation in a simplified model and also whether or not TGF-β exhibits similar proliferation effects on transformed RPE cells that it has on primary RPE cells. Furthermore, we examined the cell proliferation effects of RPE-conditioned medium (CM). A vertical wound measuring 2 mm in diameter was made on primary RPE monolayers. The expression of α-smooth muscle actin (SMA) by the cells located at the wound edges was observed using a confocal microscope under immunofluorescent staining. Cell proliferation was measured by incorporating 3H-thymidine into DNA. The presence of α-SMA was observed in the cells within the wound after treatment with TGF-β2, while negative expression was observed in control cells. TGF-βs inhibited the proliferation of the primary cultures of RPE cells in a dose-dependent manner, but the spindle-shaped late-passaged RPE cells were not inhibited by these growth factors. The medium conditioned by RPE cells stimulated the proliferation of subconjunctival fibroblasts and inhibited the proliferation of primary RPE cells, in a manner similar to TGF-β. These findings demonstrate that TGF-β-stimulated RPE cells may evoke proliferative vitreoretinopathy through mesenchymal transformation and cell proliferation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 271~277 | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Actins/analysis | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Division/drug effects | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Culture Media, Conditioned | - |
dc.subject.MESH | DNA/biosynthesis | - |
dc.subject.MESH | Mesoderm/cytology* | - |
dc.subject.MESH | Pigment Epithelium of Eye/cytology* | - |
dc.subject.MESH | Rabbits | - |
dc.subject.MESH | Swine | - |
dc.subject.MESH | Transforming Growth Factor beta/physiology* | - |
dc.subject.MESH | Vitreoretinopathy, Proliferative/etiology | - |
dc.title | TGF-βs Synthesized by RPE Cells Have Autocrine Activity on Mesenchymal Transformation and Cell Proliferation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학) | - |
dc.contributor.googleauthor | Sung Chul Lee | - |
dc.contributor.googleauthor | Soon Hyun Kim | - |
dc.contributor.googleauthor | Hyoung Jun Koh | - |
dc.contributor.googleauthor | Oh Woong Kwon | - |
dc.identifier.doi | 10.3349/ymj.2001.42.3.271 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00152 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 11456391 | - |
dc.subject.keyword | Proliferative vitreoretinopathy | - |
dc.subject.keyword | retinal pigment epithelium | - |
dc.subject.keyword | transformation | - |
dc.subject.keyword | transforming growth factor | - |
dc.contributor.alternativeName | Koh, Hyoung Jun | - |
dc.contributor.affiliatedAuthor | Koh, Hyoung Jun | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 42 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 271 | - |
dc.citation.endPage | 277 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.42(3) : 271-277, 2001 | - |
dc.identifier.rimsid | 39589 | - |
dc.type.rims | ART | - |
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