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A Plausible Method for the Diagnosis of Genetic Disorders Using Full Length cDNA
DC Field | Value | Language |
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dc.contributor.author | 김명희 | - |
dc.contributor.author | 박형우 | - |
dc.date.accessioned | 2016-02-19T11:15:37Z | - |
dc.date.available | 2016-02-19T11:15:37Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 1738-3226 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142749 | - |
dc.description.abstract | A cDNA of coagulation Factor IX gene has been screened from the $\lambda$gt11 human fetal liver cDNA library, and used to construct a 2.8-kb full length cDNA after recombining with the N-terminal fragment from pTZ-FIX. Human genomic DNA was isolated, digested with the restriction endonucleases, TaqI, EcoRI, and HindIII, and Southern hybridization was performed using the full length factor IX cDNA as a probe. The hybridized bands generated by the restriction endonucleases were the followings: TaqI, 0.3, 1.0, 1.6, 1.8, 2.7, 3.7, and 5.3 kb bands; EcoRI, 1.8, 4.8, 4.9, 5.5, 6.8, and 12.6 kb bands; HindIII, 4.1, 4.4, 5.2, 5.8, 7.6, and 12.5 kb bands. When the Southern bands were physically mapped along the genome, about 50-kb continuous region harboring almost all of the genomic region of Factor Ⅸ gene was covered. These results suggest a possibility of using an exonal cDNA probe to diagnose abnormalities including large deletions, insertions, and rearrangements along the genome, if there is any. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1~5 | - |
dc.relation.isPartOf | Journal of Experimental & Biomedcal Science (대한의생명과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | A Plausible Method for the Diagnosis of Genetic Disorders Using Full Length cDNA | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anatomy (해부학) | - |
dc.contributor.googleauthor | Hyangsuk Hur | - |
dc.contributor.googleauthor | Youngwon Lee | - |
dc.contributor.googleauthor | Hyoungwoo Park | - |
dc.contributor.googleauthor | Myoung Hee Kim | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00432 | - |
dc.contributor.localId | A01756 | - |
dc.relation.journalcode | J01408 | - |
dc.subject.keyword | full length cDNA | - |
dc.subject.keyword | diagnosis | - |
dc.subject.keyword | southern hybridization | - |
dc.contributor.alternativeName | Kim, Myoung Hee | - |
dc.contributor.alternativeName | Park, Hyoung Woo | - |
dc.contributor.affiliatedAuthor | Kim, Myoung Hee | - |
dc.contributor.affiliatedAuthor | Park, Hyoung Woo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 7 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 5 | - |
dc.identifier.bibliographicCitation | Journal of Experimental & Biomedcal Science (대한의생명과학회지), Vol.7(1) : 1-5, 2001 | - |
dc.identifier.rimsid | 29817 | - |
dc.type.rims | ART | - |
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