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TUCAN: An anti-apoptotic CARD family protein over-expressed in cancer

Authors
 Nuzhat Pathan  ;  Hiroyuki Marusawa  ;  Maryla Krajewska  ;  Shu-ichi Matsuzawa  ;  Hoguen Kim  ;  Kazuya Okada  ;  Seiji Torii  ;  Shinichi Kitada  ;  Stanislaw Krajewski  ;  Kate Welsh  ;  Frederick Pio  ;  Adam Godzik  ;  John C. Reed 
Citation
 Journal of Biological Chemistry, Vol.276(34) : 32220-32229, 2001 
Journal Title
 Journal of Biological Chemistry 
ISSN
 0021-9258 
Issue Date
2001
Abstract
Caspase-associated recruitment domains (CARDs) are protein interaction domains that participate in activation or suppression of CARD-carrying members of the caspase family of apoptosis-inducing proteases. A novel CARD-containing protein was identified that is overexpressed in some types of cancer and that binds and suppresses activation of procaspase-9, which we term TUCAN (tumor-up-regulated CARD-containing antagonist of caspase nine). The CARD domain of TUCAN selectively binds itself and procaspase-9. TUCAN interferes with binding of Apaf1 to procaspase-9 and suppresses caspase activation induced by the Apaf1 activator, cytochrome c. Overexpression of TUCAN in cells by stable or transient transfection inhibits apoptosis and caspase activation induced by Apaf1/caspase-9-dependent stimuli, including Bax, VP16, and staurosporine, but not by Apaf1/caspase-9-independent stimuli, Fas and granzyme B. High levels of endogenous TUCAN protein were detected in several tumor cell lines and in colon cancer specimens, correlating with shorter patient survival. Thus, TUCAN represents a new member of the CARD family that selectively suppresses apoptosis induced via the mitochondrial pathway for caspase activation.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/142585
DOI
10.1074/jbc.M100433200
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실)
Yonsei Authors
김호근(Kim, Ho Keun)
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