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Effect of a1-Acid Glycoprotein Expressed in Cancer Cells on Malignant Characteristics
DC Field | Value | Language |
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dc.contributor.author | 임주원 | - |
dc.date.accessioned | 2016-02-19T11:06:52Z | - |
dc.date.available | 2016-02-19T11:06:52Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142428 | - |
dc.description.abstract | The a1-acid glycoprotein (AAG) is a prototypical serum acute phase reactant in most mammalian species; it is synthesized mainly in liver parenchymal cells. Recently, we found that mRNAs of AAG were expressed in non-hepatic cancer cells, and the expression levels were regulated by the cytokines - IL-1, IL-6, and TNF-a. The functional role of AAG in non-hepatic cancer cells has not yet been established. In order to understand the functional role of the AAG expressed in HT-29 cells, the cancer cells were transfected with cloned cDNA for AAG, or exposed to antisense oligodeoxynucleotide (ODN) for AAG. The colony-forming capacity, invasion, and adhesion to laminin of these transformed cancer cells were measured. Overexpression of AAG by transfection, and inhibition of the AAG expression by antisense ODNs were identified by Western blot as well as nested reverse transcriptase-polymerase chain reaction (nested RT-PCR), respectively. Results showed that the overexpression of AAG by transfection reduced colony-forming capacities, invasion, and adhesion to laminin of the cancer cells; on the other hand, the antisense ODN for AAG elevated colony-forming capacities, invasion, and adhesion to laminin of the cancer cells. These results suggest that AAG, expressed in cancer cells inhibited proliferation, invasion, and metastasis of the cancer cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 341~345 | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Effect of a1-Acid Glycoprotein Expressed in Cancer Cells on Malignant Characteristics | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Soo Young Lee | - |
dc.contributor.googleauthor | Joo Weon Lim | - |
dc.contributor.googleauthor | You-Mie Kim | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03405 | - |
dc.relation.journalcode | J02273 | - |
dc.identifier.eissn | 0219-1032 | - |
dc.subject.keyword | α1-Acid Glycoprotein (AAG) | - |
dc.subject.keyword | Acute Phase Reactant | - |
dc.subject.keyword | Antisense | - |
dc.subject.keyword | Gene Overexpression | - |
dc.subject.keyword | Invasion | - |
dc.subject.keyword | Me-tastasis | - |
dc.subject.keyword | Proliferation | - |
dc.contributor.alternativeName | Lim, Joo Weon | - |
dc.contributor.affiliatedAuthor | Lim, Joo Weon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 11 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 341 | - |
dc.citation.endPage | 345 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, Vol.11(3) : 341-345, 2001 | - |
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