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Porphyromonas gingivalis 분쇄액으로 유도된 파골세포의 분화에 미치는 Taurine과 Alendronate의 효과
DC Field | Value | Language |
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dc.contributor.author | 이승종 | - |
dc.date.accessioned | 2016-02-19T11:06:14Z | - |
dc.date.available | 2016-02-19T11:06:14Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 1225-0864 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142403 | - |
dc.description.abstract | The objective of this study was to investigate the inhibitory effect of taurine and alendronate on the osteoclast differentiation. Osteoblasts and bone marrow cells from 1-2 day old mouse were co-cultured in 10% fetal bovine serum - minimal essential media (FBS-MEM). Osteoclast differentiation was induced by adding the sonicated extracts of Porphyromonas gingivalis (P.gingivalis). Osteoclasts were identified using tartrate resistant acid phosphotase staining (TRAP). Alendronate of 10^{-7}, 10^{-6}, 10^{-5}M and taurine of 500, 1000, 1500㎍/ml were added respectively. The cytotoxic effects of alendronate and taurine were examined using MTT(3-(4,5-dimethylthiazol -2-yl-2,5-diphenyltetrazo- lium bromide) method. After culturing with the sonicated extracts of P.gingivalis, the amounts of IL-6 in the culture supernatant were measured and compared using the ELISA method. The results were as follows : 1. Osteoclasts were differentiated at the concentration of 0.01~0.1㎍/ml sonicated extracts of P.gingivalis. (P<0.05). 2. Alendronate inhibited osteoclasts differentiation at the concentration of 10-5 M when the concentration of sonicated extracts of P.gingivalis was 0.01㎍/ml. 3. Taurine inhibited osteoclasts differentiation at the concentration of 1500㎍/ml when the concentration of sonicated extracts of P.gingivalis 0.01㎍/ml. 4. In cytotoxic test (MTT test), no cytotoxic effect was evident in all concentrations of alendronate and taurine. 5. Taurine (10-5M) and alendronate(1500㎍/ml) did not change the amounts of IL-6 induced by sonicated extracts of P.gingivalis significantly. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 285~295 | - |
dc.relation.isPartOf | Journal of Korean Academy of Operative Dentistry (대한치과보존학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Porphyromonas gingivalis 분쇄액으로 유도된 파골세포의 분화에 미치는 Taurine과 Alendronate의 효과 | - |
dc.title.alternative | THE INHIBITORY EFFECT OF TAURINE AND ALENDRONATE ON THE OSTEOCLAST DIFFERENTIATION MEDIATED BY SONICATED EXTRACTS OF PORPHYROMONAS GINGIVALIS IN VITRO. | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Conservative Dentistry (보존과학) | - |
dc.contributor.googleauthor | 박주현 | - |
dc.contributor.googleauthor | 금기연 | - |
dc.contributor.googleauthor | 이정현 | - |
dc.contributor.googleauthor | 유윤정 | - |
dc.contributor.googleauthor | 이승종 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02925 | - |
dc.relation.journalcode | J01488 | - |
dc.subject.keyword | alendronate | - |
dc.subject.keyword | taurine | - |
dc.subject.keyword | P. gingivalis | - |
dc.subject.keyword | osteoclast | - |
dc.subject.keyword | replacement root resorption | - |
dc.contributor.alternativeName | Lee, Seung Jong | - |
dc.contributor.affiliatedAuthor | Lee, Seung Jong | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 26 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 285 | - |
dc.citation.endPage | 295 | - |
dc.identifier.bibliographicCitation | Journal of Korean Academy of Operative Dentistry (대한치과보존학회지), Vol.26(4) : 285-295, 2001 | - |
dc.identifier.rimsid | 30979 | - |
dc.type.rims | ART | - |
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