113 159

Cited 0 times in

Comparison of the reverse transcription-PCR with the branched DNA assay for measurement of human immunodeficiency virus type 1 RNA levels in plasma of Korean patients

Authors
 Dong Il Won  ;  Jung Yong Park  ;  June Myung Kim  ;  Hyon Suk Kim 
Citation
 YONSEI MEDICAL JOURNAL, Vol.42(2) : 204-208, 2001 
Journal Title
YONSEI MEDICAL JOURNAL
ISSN
 0513-5796 
Issue Date
2001
Keywords
Human immunodeficiency virus(HIV) type 1 ; reverse transcription-PCR assay(RT-PCR) ; branched DNA assay(bDNA)
Abstract
Viral load testing of human immunodeficiency virus (HIV) is an essential tool for initiating and monitoring the antiretroviral therapy for HIV patients. To this end, several methods including polymerase chain reaction (PCR), branched DNA (bDNA), nucleic acid sequence based amplification assay (NASBA) and internally controlled virion PCR (ICV PCR) have become available. Of these methods, the standard reverse transcription-PCR (RT-PCR) assay has been widely used in Korea. However, no comparison study has been performed among the various detection methods currently used in Korean patients.

We evaluated the correlation and agreement between the PCR and the branched DNA (bDNA) assay for measurement of HIV RNA in Korean patients. Eighty randomly selected samples from HIV-1-seropositive patients visiting Yonsei Medical Center Severance Hospital were studied.

We found that these assays show good agreement, have a reliable correlation (r = 0.92, mean difference in log10 copies/ mL ± 2 standard deviation = 0.098 ± 0.805) and produce values whose relationship is given by the following equation: log10v3 bDNA = -0.3405 + 1.0601 · log10RT-PCR. Thus, we conclude that these two methods may allow direct comparison of the results obtained from different assay systems.
Files in This Item:
T200101637.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, June Myung(김준명)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/142128
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links