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사람 정상 코점막 상피세포에서 사이토카인에 의한 MUC8과 MUC5AC의 발현 및 조절

Other Titles
 Expression and regulation of MUC8 & MUC5AC by various cytokines in normal human nasal epithelial cells 
Authors
 이정권  ;  문형진  ;  김성식  ;  김창우  ;  윤주헌 
Citation
 Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.44(6) : 600-605, 2001 
Journal Title
Korean Journal of Otolaryngology(대한이비인후과학회지)
ISSN
 1225-035X 
Issue Date
2001
Keywords
Normal human nasal epithelial cells ; Mucin ; Cytokines ; Mucin gene 8 (MUC8) ; Mucin gene 5AC (MUC5AC)
Abstract
Background and Objectives : Sinusitis is one of the most commonly reported diseases in the world. A network of inflammatory mediators is known to be involved in the pathogenesis of chronic sinusitis and nasal mucus secretion may also be under the control of an inflammatory mediator network. To date, 12 human mucin genes have been identified; however, the regulation of MUC8 has not yet been found out. In this study, we described the regulation of the MUC8 mRNA expression by inflammatory mediators and investigated its cellular location. Materials and Method : MUC8 mRNA and MUC5AC mRNA were detected in culture using passage-2 normal human nasal epithelial(NHNE) cells after the treatment with a mixture of following inflammatory mediators; TNF-α, IL-1β, LPS, IL-4, PAF. The translocation of MUC8 mRNA from the nucleus to the cytoplasm was investigated by treating the inflammatory mediators with in situ hybridization. Results : We found that a mixture of inflammatory mediators increased the MUC8 mRNA expression but decreased the MUC5AC mRNA expression in cultured normal human nasal epithelial cells. Among the inflammatory mediators, Interleukin-4 was responsible for the decrease in the MUC5AC mRNA expression and the MUC5AC mucin secretion. We also found that MUC8 mRNA resides in the nucleus of goblet cells and is transported into the cytoplasm following the treatment with inflammatory mediators. Conclusion : These results indicate that MUC8 may play an important role in the pathogenesis of mucus hypersecretion in chronic sinusitis.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Joo Heon(윤주헌)
Lee, Jeung Gweon(이정권)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/142028
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