Cited 25 times in
A Bone Replaceable Artificial Bone Substitute: Cytotoxicity, Cell Adhesion, Proliferation, and Alkaline Phosphatase Activity
DC Field | Value | Language |
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dc.contributor.author | 박종철 | - |
dc.contributor.author | 서활 | - |
dc.date.accessioned | 2016-02-19T10:54:32Z | - |
dc.date.available | 2016-02-19T10:54:32Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0160-564X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/141968 | - |
dc.description.abstract | Cellular toxicity, cell adhesion and proliferation, and alkaline phosphatase (ALP) activity were investigated for an artificial bone substitute composed of heated carbonate apatite (CAp) and Type I atelocollagen (AtCol) extracted from bovine tail skins (88/12 in %wt/wt). To enhance the intramolecular crosslinking between collagen molecules, the CAp-AtCol substitutes were irradiated by ultraviolet rays (wave length 254 nm) at 4°C for 4 h or vacuum dried at 150°C for 2 h. Cytotoxicity tests by a direct contact method and an extract dilution method revealed that the CAp-AtCol substitutes were cytocompatible for balb 3T3 fibroblasts. Osteoblast adhesion studies demonstrated that the substitute disks composed of 980°C-heated CAp and AtCol were significantly more adhesive for osteoblasts than those of 1,200°C-sintered CAp and AtCol (p < 0.05). Proliferation studies showed that the number of osteoblasts grown in the media containing substitutes of 980°C-heated CAp and AtCol was statistically higher than grown in those of 1,200°C-sintered CAp and AtCol after 5 days (p < 0.05). It was found that osteoblasts grown in the substitutes of 980°C-heated CAp and AtCol only expressed similar ALP activity to the controls. These results suggested that the substitutes consisting of 980°C-heated CAp and AtCol show more favorable interactions with osteoblasts than those of 1,200°C-sintered CAp and AtCol. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 14~21 | - |
dc.relation.isPartOf | ARTIFICIAL ORGANS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | A Bone Replaceable Artificial Bone Substitute: Cytotoxicity, Cell Adhesion, Proliferation, and Alkaline Phosphatase Activity | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Medical Engineering (의학공학) | - |
dc.contributor.googleauthor | Hwal Suh | - |
dc.contributor.googleauthor | Jong-Chul Park | - |
dc.contributor.googleauthor | Dong-Wook Han | - |
dc.contributor.googleauthor | Dong Hee Lee | - |
dc.contributor.googleauthor | Chang Dong Han | - |
dc.identifier.doi | 10.1046/j.1525-1594.2001.025001014.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01662 | - |
dc.contributor.localId | A01924 | - |
dc.relation.journalcode | J00246 | - |
dc.identifier.eissn | 1525-1594 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1046/j.1525-1594.2001.025001014.x/abstract | - |
dc.subject.keyword | Carbonate apatite-Type latelocollagen substitute | - |
dc.subject.keyword | Cytotoxicity | - |
dc.subject.keyword | Osteoblast adhesion | - |
dc.subject.keyword | Oseoblast proliferation | - |
dc.subject.keyword | Alkaline phosphatase activity | - |
dc.contributor.alternativeName | Park, Jong Chul | - |
dc.contributor.alternativeName | Suh, Hwal | - |
dc.contributor.affiliatedAuthor | Park, Jong Chul | - |
dc.contributor.affiliatedAuthor | Suh, Hwal | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 25 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 14 | - |
dc.citation.endPage | 21 | - |
dc.identifier.bibliographicCitation | ARTIFICIAL ORGANS, Vol.25(1) : 14-21, 2001 | - |
dc.identifier.rimsid | 35889 | - |
dc.type.rims | ART | - |
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