269 308

Cited 1 times in

Biocompatibility Evaluation of Dental Luting Cements Using Cytokine Released from Human Oral Fibroblasts and Keratinocytes

 Jae-Sung Kwon  ;  Yin-Zhu Piao  ;  Sun-A Cho  ;  Song-Yi Yang  ;  Ji Hoon Kim  ;  Susun An  ;  Kwang-Mahn Kim 
 MATERIALS, Vol.8(11) : 7269-7277, 2015 
Journal Title
Issue Date
biocompatibility ; cytokine ; cytotoxicity ; dental luting cement ; fibroblast ; inflammation ; keratinocyte
Dental luting cements are commonly used in dentistry for cementation of prosthetic restoration. Many previous studies focused on the measurement of the cell viability as the method of cytotoxicity evaluation during biocompatibility study for the material. In this study, the biocompatibility of various dental luting cements were evaluated using the new method of cytokine release measurement in order to better simulate inflammatory reactions in animal or clinical model using two different oral cells; immortalized human gingival fibroblast and immortalized human oral keratinocytes. Cells were exposed to extractions of various commercially available dental luting cements for different durations. Cytokines of IL-1α and IL-8 were measured from the supernatants of the cells and the results were then compared to the conventional MTT viability test. The result from the conventional cell viability study showed a relatively simple and straight forward indication that only one of the dental luting cements tested in this study was cytotoxic with increasing duration of exposure for both cells. Meanwhile, the result from the cytokine measurement study was much more complex at the time point they were measured, type of cells used for the study and the type of cytokines measured, all of which influenced the interpretation of the results. Hence, the better understanding of the cytokine release would be required for the application in biocompatibility evaluation.
Files in This Item:
T201504331.pdf Download
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Jae-Sung(권재성) ORCID logo https://orcid.org/0000-0001-9803-7730
Kim, Kwang Mahn(김광만) ORCID logo https://orcid.org/0000-0002-5235-0294
Yang, Song Yi(양송이)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.