Cited 18 times in
Conventional and real-time PCR targeting 16S ribosomal RNA for the detection of Mycobacterium tuberculosis complex
DC Field | Value | Language |
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dc.contributor.author | 조상래 | - |
dc.date.accessioned | 2016-02-04T12:02:19Z | - |
dc.date.available | 2016-02-04T12:02:19Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1027-3719 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/141753 | - |
dc.description.abstract | SETTING: Conventional diagnostic methods for tuberculosis (TB) have limited sensitivity and specificity or are time-consuming. OBJECTIVE: 16S rDNA and 16S rRNA of Mycobacterium tuberculosis complex (MTC) were used as targets to develop sensitive and specific polymerase chain reactions (PCRs) to improve the diagnosis of MTC. DESIGN: We developed conventional and real-time PCRs targeting 16S rDNA and rRNA of MTC. RESULTS: PCRs targeting 16S rRNA had a 10-100 times lower limit of detection for M. tuberculosis than PCRs targeting 16S rDNA. The sensitivities of the 16S rDNA PCR, 16S rRNA reverse transcription PCR (RT-PCR), 16S rDNA real-time PCR and 16S rRNA real-time RT-PCR for sputum specimens were respectively 92%, 94.6%, 96% and 100%. Real-time PCR showed no cross-reactivity, but conventional PCR had cross-reactivity to M. avium, M. gastri and M. nonchromogenicum. CONCLUSION: PCRs targeting the 16S rRNA of MTC were more sensitive than those targeting 16S rDNA; 16S rRNA real-time RT-PCR showed the highest sensitivity and specificity for MTC. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1102~1108 | - |
dc.language | English | - |
dc.publisher | The Union | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Bacteriological Techniques/methods* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mycobacterium tuberculosis/genetics | - |
dc.subject.MESH | Mycobacterium tuberculosis/isolation & purification* | - |
dc.subject.MESH | RNA, Bacterial/genetics | - |
dc.subject.MESH | RNA, Ribosomal, 16S/genetics | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction/methods* | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | Sputum/microbiology* | - |
dc.subject.MESH | Tuberculosis/diagnosis* | - |
dc.title | Conventional and real-time PCR targeting 16S ribosomal RNA for the detection of Mycobacterium tuberculosis complex | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Choi, Y. | - |
dc.contributor.googleauthor | Hong, S-R. | - |
dc.contributor.googleauthor | Jeon, B-Y. | - |
dc.contributor.googleauthor | Wang, H-Y. | - |
dc.contributor.googleauthor | Lee, G-S. | - |
dc.contributor.googleauthor | Cho, S-N. | - |
dc.contributor.googleauthor | Shim, T. S. | - |
dc.contributor.googleauthor | Lee, H. | - |
dc.identifier.doi | 10.5588/ijtld.14.0472 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03824 | - |
dc.relation.journalcode | J01167 | - |
dc.identifier.eissn | 1815-7920 | - |
dc.identifier.pmid | 26260833 | - |
dc.identifier.url | http://www.ingentaconnect.com/content/iuatld/ijtld/2015/00000019/00000009/art00020 | - |
dc.subject.keyword | MTC | - |
dc.subject.keyword | 16S rDNA | - |
dc.subject.keyword | 16S rRNA | - |
dc.subject.keyword | conventional polymerase chain reaction | - |
dc.subject.keyword | real-time PCR | - |
dc.contributor.alternativeName | Cho, Sang Nae | - |
dc.contributor.affiliatedAuthor | Cho, Sang Nae | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 19 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1102 | - |
dc.citation.endPage | 1108 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE, Vol.19(9) : 1102-1108, 2015 | - |
dc.identifier.rimsid | 30865 | - |
dc.type.rims | ART | - |
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