Cited 92 times in
Preparation of a cyanine-based fluorescent probe for highly selective detection of glutathione and its use in living cells and tissues of mice
DC Field | Value | Language |
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dc.contributor.author | 유지환 | - |
dc.date.accessioned | 2016-02-04T11:56:35Z | - |
dc.date.available | 2016-02-04T11:56:35Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1754-2189 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/141543 | - |
dc.description.abstract | Glutathione (GSH) is a major endogenous antioxidant that has a central role in cellular defense against toxins and free radicals. This protocol describes the preparation of CPDSA, a cyanine-based near-infrared (NIR) fluorescent probe for the detection of GSH in cells and in vivo. CPDSA is prepared with high yield through a simple two-step process. The first step is to react commercially available IR-780 iodide with excess anhydrous piperazine in anhydrous N,N-dimethyl formamide at 85 °C to form cyanine-piperazine (CP). The second step is the sulfonylation of CP with dansyl chloride in anhydrous dichloromethane. CPDSA selectively detects GSH in cells, and it has been shown to not react with other biothiols such as cysteine (Cys) and homocysteine (Hcy). This probe can also be used to monitor the GSH level of mouse bone marrow-derived neutrophils (BMDNs). The preparation of probe CPDSA takes 2 d, and experiments in cells and mice take 12-13 d. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1742~1754 | - |
dc.relation.isPartOf | NATURE PROTOCOLS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Carbocyanines/metabolism* | - |
dc.subject.MESH | Cytological Techniques/methods* | - |
dc.subject.MESH | Fluorescent Dyes/chemical synthesis* | - |
dc.subject.MESH | Fluorescent Dyes/isolation & purification* | - |
dc.subject.MESH | Glutathione/analysis* | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.title | Preparation of a cyanine-based fluorescent probe for highly selective detection of glutathione and its use in living cells and tissues of mice | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Research Center for Human Natural Defense System (생체방어연구센터) | - |
dc.contributor.googleauthor | Jun Yin | - |
dc.contributor.googleauthor | Younghee Kwon | - |
dc.contributor.googleauthor | Dabin Kim | - |
dc.contributor.googleauthor | Dayoung Lee | - |
dc.contributor.googleauthor | Gyoungmi Kim | - |
dc.contributor.googleauthor | Ying Hu | - |
dc.contributor.googleauthor | Ji-Hwan Ryu | - |
dc.contributor.googleauthor | Juyoung Yoon | - |
dc.identifier.doi | 10.1038/nprot.2015.109 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02522 | - |
dc.relation.journalcode | J02300 | - |
dc.identifier.eissn | 1750-2799 | - |
dc.identifier.pmid | 26492135 | - |
dc.identifier.url | http://www.nature.com/nprot/journal/v10/n11/full/nprot.2015.109.html | - |
dc.contributor.alternativeName | Ryu, Ji Hwan | - |
dc.contributor.affiliatedAuthor | Ryu, Ji Hwan | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 10 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1742 | - |
dc.citation.endPage | 1754 | - |
dc.identifier.bibliographicCitation | NATURE PROTOCOLS, Vol.10(11) : 1742-1754, 2015 | - |
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