Cited 30 times in
Effective Gene Delivery into Human Stem Cells with a Cell-Targeting Peptide-Modified Bioreducible Polymer
DC Field | Value | Language |
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dc.contributor.author | 김형범 | - |
dc.date.accessioned | 2016-02-04T11:54:46Z | - |
dc.date.available | 2016-02-04T11:54:46Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1613-6810 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/141474 | - |
dc.description.abstract | Stem cells are poorly permissive to non-viral gene transfection reagents. In this study, we explored the possibility of improving gene delivery into human embryonic (hESC) and mesenchymal (hMSC) stem cells by synergizing the activity of a cell-binding ligand with a polymer that releases nucleic acids in a cytoplasm-responsive manner. A 29 amino acid long peptide, RVG, targeting the nicotinic acetylcholine receptor (nAchR) was identified to bind both hMSC and H9-derived hESC. Conjugating RVG to a redox-sensitive biodegradable dendrimer-type arginine-grafted polymer (PAM-ABP) enabled nanoparticle formation with plasmid DNA without altering the environment-sensitive DNA release property and favorable toxicity profile of the parent polymer. Importantly, RVG-PAM-ABP quantitatively enhanced transfection into both hMSC and hESC compared to commercial transfection reagents like Lipofectamine 2000 and Fugene. ∼60% and 50% of hMSC and hESC were respectively transfected, and at increased levels on a per cell basis, without affecting pluripotency marker expression. RVG-PAM-ABP is thus a novel bioreducible, biocompatible, non-toxic, synthetic gene delivery system for nAchR-expressing stem cells. Our data also demonstrates that a cell-binding ligand like RVG can cooperate with a gene delivery system like PAM-ABP to enable transfection of poorly-permissive cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 2069~2079 | - |
dc.relation.isPartOf | SMALL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Arginine/chemistry | - |
dc.subject.MESH | Biocompatible Materials/chemistry* | - |
dc.subject.MESH | Cytoplasm/metabolism | - |
dc.subject.MESH | DNA/chemistry | - |
dc.subject.MESH | Embryonic Stem Cells/cytology | - |
dc.subject.MESH | Fibroblasts/metabolism | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | Gene Transfer Techniques* | - |
dc.subject.MESH | Genetic Vectors/chemistry* | - |
dc.subject.MESH | Green Fluorescent Proteins/chemistry | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Ligands | - |
dc.subject.MESH | Lipids/chemistry | - |
dc.subject.MESH | Mesenchymal Stromal Cells/cytology | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Oxidation-Reduction | - |
dc.subject.MESH | Peptides/chemistry* | - |
dc.subject.MESH | Phenotype | - |
dc.subject.MESH | Plasmids/metabolism | - |
dc.subject.MESH | Polymers/chemistry | - |
dc.subject.MESH | Receptors, Nicotinic/metabolism | - |
dc.subject.MESH | Stem Cells/cytology* | - |
dc.subject.MESH | Transfection | - |
dc.title | Effective Gene Delivery into Human Stem Cells with a Cell-Targeting Peptide-Modified Bioreducible Polymer | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Jagadish Beloor | - |
dc.contributor.googleauthor | Suresh Ramakrishna | - |
dc.contributor.googleauthor | Kihoon Nam | - |
dc.contributor.googleauthor | Chang Seon Choi | - |
dc.contributor.googleauthor | Jongkil Kim | - |
dc.contributor.googleauthor | Sung Hwa Kim | - |
dc.contributor.googleauthor | Hyong Jin Cho | - |
dc.contributor.googleauthor | HeungSoo Shin | - |
dc.contributor.googleauthor | Hyongbum Kim | - |
dc.contributor.googleauthor | Sung Wan Kim | - |
dc.contributor.googleauthor | Sang-Kyung Lee | - |
dc.contributor.googleauthor | Priti Kumar | - |
dc.identifier.doi | 10.1002/smll.201402933 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01148 | - |
dc.relation.journalcode | J02664 | - |
dc.identifier.eissn | 1613-6829 | - |
dc.identifier.pmid | 25515928 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/smll.201402933/full | - |
dc.subject.keyword | bioreducible polymer | - |
dc.subject.keyword | cell-binding ligand | - |
dc.subject.keyword | embryonic stem cells | - |
dc.subject.keyword | mesenchymal stem cells | - |
dc.subject.keyword | non-viral gene delivery | - |
dc.contributor.alternativeName | Kim, Hyongbum | - |
dc.contributor.affiliatedAuthor | Kim, Hyongbum | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 11 | - |
dc.citation.number | 17 | - |
dc.citation.startPage | 2069 | - |
dc.citation.endPage | 2079 | - |
dc.identifier.bibliographicCitation | SMALL, Vol.11(17) : 2069-2079, 2015 | - |
dc.identifier.rimsid | 30668 | - |
dc.type.rims | ART | - |
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