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Effective Gene Delivery into Human Stem Cells with a Cell-Targeting Peptide-Modified Bioreducible Polymer

DC FieldValueLanguage
dc.contributor.author김형범-
dc.date.accessioned2016-02-04T11:54:46Z-
dc.date.available2016-02-04T11:54:46Z-
dc.date.issued2015-
dc.identifier.issn1613-6810-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/141474-
dc.description.abstractStem cells are poorly permissive to non-viral gene transfection reagents. In this study, we explored the possibility of improving gene delivery into human embryonic (hESC) and mesenchymal (hMSC) stem cells by synergizing the activity of a cell-binding ligand with a polymer that releases nucleic acids in a cytoplasm-responsive manner. A 29 amino acid long peptide, RVG, targeting the nicotinic acetylcholine receptor (nAchR) was identified to bind both hMSC and H9-derived hESC. Conjugating RVG to a redox-sensitive biodegradable dendrimer-type arginine-grafted polymer (PAM-ABP) enabled nanoparticle formation with plasmid DNA without altering the environment-sensitive DNA release property and favorable toxicity profile of the parent polymer. Importantly, RVG-PAM-ABP quantitatively enhanced transfection into both hMSC and hESC compared to commercial transfection reagents like Lipofectamine 2000 and Fugene. ∼60% and 50% of hMSC and hESC were respectively transfected, and at increased levels on a per cell basis, without affecting pluripotency marker expression. RVG-PAM-ABP is thus a novel bioreducible, biocompatible, non-toxic, synthetic gene delivery system for nAchR-expressing stem cells. Our data also demonstrates that a cell-binding ligand like RVG can cooperate with a gene delivery system like PAM-ABP to enable transfection of poorly-permissive cells.-
dc.description.statementOfResponsibilityopen-
dc.format.extent2069~2079-
dc.relation.isPartOfSmall-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleEffective Gene Delivery into Human Stem Cells with a Cell-Targeting Peptide-Modified Bioreducible Polymer-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학)-
dc.contributor.googleauthorJagadish Beloor-
dc.contributor.googleauthorSuresh Ramakrishna-
dc.contributor.googleauthorKihoon Nam-
dc.contributor.googleauthorChang Seon Choi-
dc.contributor.googleauthorJongkil Kim-
dc.contributor.googleauthorSung Hwa Kim-
dc.contributor.googleauthorHyong Jin Cho-
dc.contributor.googleauthorHeungSoo Shin-
dc.contributor.googleauthorHyongbum Kim-
dc.contributor.googleauthorSung Wan Kim-
dc.contributor.googleauthorSang-Kyung Lee-
dc.contributor.googleauthorPriti Kumar-
dc.identifier.doi10.1002/smll.201402933-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01148-
dc.relation.journalcodeJ02664-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/smll.201402933/full-
dc.contributor.alternativeNameKim, Hyongbum-
dc.contributor.affiliatedAuthorKim, Hyongbum-
dc.rights.accessRightsnot free-
dc.citation.volume11-
dc.citation.number17-
dc.citation.startPage2069-
dc.citation.endPage2079-
dc.identifier.bibliographicCitationSmall, Vol.11(17) : 2069-2079, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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