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RAGE siRNA-mediated gene silencing provides cardioprotection against ventricular arrhythmias in acute ischemia and reperfusion

DC FieldValueLanguage
dc.contributor.author김동규-
dc.contributor.author박혜림-
dc.contributor.author박혜원-
dc.contributor.author박희남-
dc.contributor.author이문형-
dc.contributor.author정보영-
dc.contributor.author최동훈-
dc.contributor.author홍주은-
dc.date.accessioned2016-02-04T11:52:19Z-
dc.date.available2016-02-04T11:52:19Z-
dc.date.issued2015-
dc.identifier.issn0168-3659-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/141387-
dc.description.abstractExpression of receptor for advanced glycation end-products (RAGE) is suggested to play a crucial role in mediating cardiac ischemia/reperfusion (IR) injury, and the blockade of RAGE signaling has been considered as a potential therapeutic strategy for the treatment of IR-induced cardiac damage. In this study, we primarily investigated the effects of RAGE suppression particularly on IR-induced ventricular arrhythmia. To inhibit the IR-induced upregulation of RAGE, siRNA targeting RAGE (siRAGE) was delivered to myocardium by using deoxycholic acid-modified polyethylenimine (PEI-DA) as a non-viral gene carrier. The resultant PEI-DA/siRAGE nanocomplexes successfully silenced the expression of RAGE and attenuated the inflammation and apoptosis in the ischemic-reperfused myocardium. According to our results, the electrophysiological properties (e.g., action potential propagation, action potential duration, and conduction velocity), disrupted by IR injury, were restored to normal level and the induction of ventricular tachycardia was abolished by RAGE silencing. We further found that RAGE suppression led to the activation of Wnt signaling, followed by the expression of gap junction protein, connexin43. Thus it could be concluded that successful siRAGE delivery is protective against IR-induced ventricular arrhythmia.-
dc.description.statementOfResponsibilityopen-
dc.format.extent315~326-
dc.relation.isPartOfJOURNAL OF CONTROLLED RELEASE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHArrhythmias, Cardiac/etiology-
dc.subject.MESHArrhythmias, Cardiac/metabolism-
dc.subject.MESHArrhythmias, Cardiac/therapy*-
dc.subject.MESHCell Line-
dc.subject.MESHConnexin 43/metabolism-
dc.subject.MESHGene Transfer Techniques-
dc.subject.MESHMale-
dc.subject.MESHMyocardial Reperfusion Injury/complications-
dc.subject.MESHMyocardial Reperfusion Injury/metabolism-
dc.subject.MESHMyocardial Reperfusion Injury/therapy*-
dc.subject.MESHMyocardium/metabolism-
dc.subject.MESHRNA, Small Interfering/administration & dosage*-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHReceptor for Advanced Glycation End Products/genetics*-
dc.subject.MESHWnt Signaling Pathway-
dc.titleRAGE siRNA-mediated gene silencing provides cardioprotection against ventricular arrhythmias in acute ischemia and reperfusion-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.googleauthorHyelim Park-
dc.contributor.googleauthorSook Hee Ku-
dc.contributor.googleauthorHyewon Park-
dc.contributor.googleauthorJueun Hong-
dc.contributor.googleauthorDongkyu Kim-
dc.contributor.googleauthorBum-Rak Choi-
dc.contributor.googleauthorHui-Nam Pak-
dc.contributor.googleauthorMoon-Hyoung Lee-
dc.contributor.googleauthorHyejung Mok-
dc.contributor.googleauthorJi Hoon Jeong-
dc.contributor.googleauthorDonghoon Choi-
dc.contributor.googleauthorSun Hwa Kim-
dc.contributor.googleauthorBoyoung Joung-
dc.identifier.doi10.1016/j.jconrel.2015.09.006-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00397-
dc.contributor.localIdA01760-
dc.contributor.localIdA01767-
dc.contributor.localIdA01776-
dc.contributor.localIdA02766-
dc.contributor.localIdA03609-
dc.contributor.localIdA04053-
dc.contributor.localIdA04437-
dc.relation.journalcodeJ01352-
dc.identifier.eissn1873-4995-
dc.identifier.pmid26381899-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0168365915301085-
dc.subject.keywordArrhythmia-
dc.subject.keywordConnexin43-
dc.subject.keywordIschemia/reperfusion injury-
dc.subject.keywordReceptor for advanced glycation end-products-
dc.subject.keywordWnt1-
dc.subject.keywordsiRNA-
dc.contributor.alternativeNameKim, Dong Kyu-
dc.contributor.alternativeNamePark, Hye Lim-
dc.contributor.alternativeNamePark, Hye Won-
dc.contributor.alternativeNamePak, Hui Nam-
dc.contributor.alternativeNameLee, Moon Hyoung-
dc.contributor.alternativeNameJoung, Bo Young-
dc.contributor.alternativeNameChoi, Dong Hoon-
dc.contributor.alternativeNameHong, Ju Eun-
dc.contributor.affiliatedAuthorKim, Dong Kyu-
dc.contributor.affiliatedAuthorPark, Hye Lim-
dc.contributor.affiliatedAuthorPark, Hye Won-
dc.contributor.affiliatedAuthorPak, Hui Nam-
dc.contributor.affiliatedAuthorLee, Moon Hyoung-
dc.contributor.affiliatedAuthorJoung, Bo Young-
dc.contributor.affiliatedAuthorChoi, Dong Hoon-
dc.contributor.affiliatedAuthorHong, Ju Eun-
dc.rights.accessRightsnot free-
dc.citation.volume217-
dc.citation.startPage315-
dc.citation.endPage326-
dc.identifier.bibliographicCitationJOURNAL OF CONTROLLED RELEASE, Vol.217 : 315-326, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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