0 115

Multimodal imaging probe for targeting cancer cells using uMUC-1 aptamer

Authors
 Won Jun Kang  ;  Jonghwan Lee  ;  Yong Seung Lee  ;  Sujeong Cho  ;  Bahy A. Ali  ;  Abdulaziz A. Al-Khedhairy  ;  Hyejung Heo  ;  Soonhag Kim 
Citation
 Colloids and Surfaces B: Biointerfaces, Vol.136 : 134-140, 2015 
Journal Title
 Colloids and Surfaces B: Biointerfaces 
ISSN
 0927-7765 
Issue Date
2015
Abstract
For adequate cancer therapy, newer imaging modalities with more specific ligands for unique targets are crucial. Underglycosylated mucin-1 (uMUC-1) antigen is an early marker of tumor development and is widely overexpressed on most tumors. A combination of nanotechnology with optical, radionuclide, and magnetic resonance (MR) imaging has great potential to improve cancer diagnosis and therapy. In this study, a multimodal nanoparticle imaging system was developed that can be used for optical, MR and positron emission tomography (PET) imaging. Cobalt ferrite magnetic nanoparticles surrounded by fluorescent rhodamine (designated MF) within a silica shell matrix were conjugated with an aptamer targeting uMUC-1 (designated MF-uMUC-1) and further labeled by 68Ga (designated MFR-uMUC-1) with the help of a p-SCN-bn-NOTA chelating agent, resulting in single multimodal nanoparticles. The resultant nanoparticles are spherical and monodispersed, as revealed by transmission electron microscopy. The MFR-uMUC-1 nanoparticle showed specific and dose-dependent fluorescent, radioisotope and MR signals targeting BT-20 cells expressing uMUC-1. In vivo targeting and multimodal imaging in tumor-bearing nude mice also showed great specificity for targeting cancers with MFR-uMUC-1. The MFR-uMUC-1 probe could be used as a single multimodal probe to visualize cancer cells by means of optical, radionuclide and MR imaging.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/141192
Full Text
http://www.sciencedirect.com/science/article/pii/S0927776515301739
DOI
10.1016/j.colsurfb.2015.09.004
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Nuclear Medicine (핵의학교실)
Yonsei Authors
강원준(Kang, Won Jun)
Export
RIS (EndNote)
XLS (Excel)
XML
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse