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Effect of eicosapentaenoic acid on cholesterol gallstone formation in C57BL/6J mice

 Soo‑Min Cho  ;  Jin‑A Park  ;  Na‑Hyun Kim  ;  Dong‑Soon Kim  ;  Dan Zhang  ;  Hee Yi  ;  Hee‑Jung Cho  ;  Ja Kyung Kim  ;  Dong Ki Lee  ;  Jin‑Suk Kim  ;  Ho‑Chul Shin 
 MOLECULAR MEDICINE REPORTS, Vol.11(1) : 362-366, 2015 
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Animals ; Cholesterol/chemistry ; Cholesterol/metabolism* ; Disease Models, Animal ; Docosahexaenoic Acids/administration & dosage ; Docosahexaenoic Acids/pharmacology ; Eicosapentaenoic Acid/administration & dosage ; Eicosapentaenoic Acid/pharmacology* ; Gallstones/chemistry ; Gallstones/drug therapy ; Gallstones/etiology* ; Gallstones/metabolism* ; Gallstones/pathology ; Gene Expression Regulation/drug effects ; Hydroxymethylglutaryl CoA Reductases/genetics ; Hydroxymethylglutaryl CoA Reductases/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Mucins/genetics ; Mucins/metabolism ; Receptors, LDL/genetics ; Receptors, LDL/metabolism
The present study investigated the preventive effect of ω-3 fatty acids against cholesterol gallstone (CG) formation. CG formation was induced in C57BL/6J mice using a lithogenic diet (LD). The mice were divided into four treatment groups: i) LD, ii) LD plus eicosapentaenoic acid (EPA), iii) LD plus docosahexaenoic acid (DHA) and iv) LD plus EPA plus DHA. Subsequent to feeding the mice the LD for four weeks, EPA and/or DHA (70 mg/kg/day) were orally administered for eight weeks. The mice in the EPA treatment groups exhibited significantly less gallstone formation than those in the LD group. By contrast, DHA treatment only slightly suppressed gallstone formation. The expression of mucin 2, 5AC, 5B and 6 was significantly decreased in the gallbladders of mice in the EPA groups (70-90%) and the LD plus DHA group (30-50%), compared with that in the mice in the LD group. In addition, the mRNA expression of 3-hydroxy-3-methylglutaryl-coenzyme A reductase was significantly decreased in the livers of mice in the EPA treatment group compared with that in the livers of mice in the LD group. In conclusion, EPA was found to have a dominant anti-lithogenic effect in C57BL/6J mice.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ja Kyung(김자경) ORCID logo https://orcid.org/0000-0001-5025-6846
Lee, Dong Ki(이동기) ORCID logo https://orcid.org/0000-0002-0048-9112
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