Cited 17 times in
Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model
DC Field | Value | Language |
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dc.contributor.author | 이원재 | - |
dc.contributor.author | 이주희 | - |
dc.contributor.author | 김용욱 | - |
dc.contributor.author | 송승용 | - |
dc.contributor.author | 유대현 | - |
dc.date.accessioned | 2016-02-04T11:44:14Z | - |
dc.date.available | 2016-02-04T11:44:14Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0032-1052 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/141085 | - |
dc.description.abstract | BACKGROUND: The 90-kDa heat-shock protein (heat-shock protein 90) is an abundant cytosolic chaperone, and inhibition of heat-shock protein 90 by 17-allylamino-17-demethoxygeldanamycin (17-AAG) compromises transforming growth factor (TGF)-β-mediated transcriptional responses by enhancing TGF-β receptor I and II degradation, thus preventing Smad2/3 activation. In this study, the authors evaluated whether heat-shock protein 90 regulates TGF-β signaling in the pathogenesis and treatment of keloids. METHODS: Keloid fibroblasts were treated with 17-AAG (10 μM), and mRNA levels of collagen types I and III were determined by real-time reverse- transcriptase polymerase chain reaction. Also, secreted TGF-β1 was assessed by enzyme-linked immunosorbent assay. The effect of 17-AAG on protein levels of Smad2/3 complex was determined by Western blot analysis. In addition, in 17-AAG-treated keloid spheroids, the collagen deposition and expression of major extracellular matrix proteins were investigated by means of Masson trichrome staining and immunohistochemistry. RESULTS: The authors found that heat-shock protein 90 is overexpressed in human keloid tissue compared with adjacent normal tissue, and 17-AAG decreased mRNA levels of type I collagen, secreted TGF-ß1, and Smad2/3 complex protein expression in keloid fibroblasts. Masson trichrome staining revealed that collagen deposition was decreased in 17-AAG-treated keloid spheroids, and immunohistochemical analysis showed that expression of collagen types I and III, elastin, and fibronectin was markedly decreased in 17-AAG-treated keloid spheroids. CONCLUSION: These results suggest that the antifibrotic action of heat-shock protein 90 inhibitors such as 17-AAG may have therapeutic effects on keloids. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 328e~337e | - |
dc.relation.isPartOf | PLASTIC AND RECONSTRUCTIVE SURGERY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Benzoquinones/pharmacology* | - |
dc.subject.MESH | Benzoquinones/therapeutic use | - |
dc.subject.MESH | Biomarkers/metabolism | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Collagen Type I/metabolism* | - |
dc.subject.MESH | Collagen Type III/metabolism* | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay | - |
dc.subject.MESH | Extracellular Matrix/drug effects* | - |
dc.subject.MESH | Extracellular Matrix/metabolism | - |
dc.subject.MESH | Fibroblasts/drug effects* | - |
dc.subject.MESH | Fibroblasts/metabolism | - |
dc.subject.MESH | HSP90 Heat-Shock Proteins/antagonists & inhibitors* | - |
dc.subject.MESH | HSP90 Heat-Shock Proteins/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Keloid/drug therapy | - |
dc.subject.MESH | Keloid/metabolism* | - |
dc.subject.MESH | Keloid/pathology | - |
dc.subject.MESH | Lactams, Macrocyclic/pharmacology* | - |
dc.subject.MESH | Lactams, Macrocyclic/therapeutic use | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Spheroids, Cellular/drug effects | - |
dc.subject.MESH | Spheroids, Cellular/metabolism | - |
dc.subject.MESH | Spheroids, Cellular/pathology | - |
dc.subject.MESH | Transforming Growth Factor beta/antagonists & inhibitors | - |
dc.subject.MESH | Transforming Growth Factor beta/metabolism | - |
dc.title | Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Plastic Surgery & Reconstructive Surgery (성형외과학) | - |
dc.contributor.googleauthor | Lee, Won Jai | - |
dc.contributor.googleauthor | Lee, Ju Hee | - |
dc.contributor.googleauthor | Ahn, Hyo Min | - |
dc.contributor.googleauthor | Song, Seung Yong | - |
dc.contributor.googleauthor | Kim, Yong Oock | - |
dc.contributor.googleauthor | Lew, Dae Hyun | - |
dc.contributor.googleauthor | Yun, Chae-Ok | - |
dc.identifier.doi | 10.1097/PRS.0000000000001538 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03005 | - |
dc.contributor.localId | A00749 | - |
dc.contributor.localId | A02032 | - |
dc.contributor.localId | A02459 | - |
dc.contributor.localId | A03171 | - |
dc.relation.journalcode | J02534 | - |
dc.identifier.eissn | 1529-4242 | - |
dc.identifier.pmid | 26313837 | - |
dc.identifier.url | http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00006534-201509000-00015&LSLINK=80&D=ovft | - |
dc.contributor.alternativeName | Lee, Won Jai | - |
dc.contributor.alternativeName | Lee, Ju Hee | - |
dc.contributor.alternativeName | Kim, Yong Oock | - |
dc.contributor.alternativeName | Song, Seung Yong | - |
dc.contributor.alternativeName | Lew, Dae Hyun | - |
dc.contributor.affiliatedAuthor | Lee, Won Jai | - |
dc.contributor.affiliatedAuthor | Kim, Yong Oock | - |
dc.contributor.affiliatedAuthor | Song, Seung Yong | - |
dc.contributor.affiliatedAuthor | Lew, Dae Hyun | - |
dc.contributor.affiliatedAuthor | Lee, Ju Hee | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 136 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 328 | - |
dc.citation.endPage | 337 | - |
dc.identifier.bibliographicCitation | PLASTIC AND RECONSTRUCTIVE SURGERY, Vol.136(3) : 328-337, 2015 | - |
dc.identifier.rimsid | 30501 | - |
dc.type.rims | ART | - |
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