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The mRNA and Protein Levels of Tubulin and β-Actin Are Greatly Reduced in the Proximal Duodenum of Mice Relative to the Rest of the Small Intestines

Authors
 Sungsook Yu  ;  Hyekyung E. Hwang  ;  Nakhyeon Yun  ;  James R. Goldenring  ;  Ki Taek Nam 
Citation
 Digestive Diseases and Sciences, Vol.60(9) : 2670-2676, 2015 
Journal Title
 Digestive Diseases and Sciences 
ISSN
 0163-2116 
Issue Date
2015
Abstract
To accurately quantify mRNA and protein levels, it is critical to choose appropriate internal standards. As the expression of housekeeping genes is assumed to remain constant, they are often employed to normalize signals to correct for sample-to-sample variations. However, recent studies have documented that β-actin and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression levels change in response to various stimuli during proliferation, activation, and differentiation. We investigated levels of α-, β-, γ-tubulin, β-actin, and GAPDH vary across the gastrointestinal tract of mice. We found that different regions of the small intestines had dramatically different expression profiles, as measured by western blot, quantitative Reverse transcription polymerase chain reaction (RT-PCR), and immunohistochemical staining. These results revealed that the expression levels of tubulins and β-actin were dramatically lower in the proximal duodenum, relative to the rest of the small intestines. These varying levels of housekeeping genes may reflect differences in the activities of specialized tissues and suggest unique requirements for tubulins in these tissue types. We conclude that the use of a single housekeeping gene to normalize gene expression in the gastrointestinal tracts of mice may introduce errors, as measured differences in gene expression may reflect regulation of the internal control rather than the mRNA or protein under investigation.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140950
DOI
10.1007/s10620-015-3688-7
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부)
Yonsei Authors
남기택(Nam, Ki Taek) ; 유성숙(Yu, Sungsook)
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Full Text
http://link.springer.com/article/10.1007%2Fs10620-015-3688-7
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