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Sound packing DNA: packing open circular DNA with low-intensity ultrasound

Authors
 DongHee Park  ;  Bong-Kwang Jung  ;  Hyunjin Park  ;  Hyungbeen Lee  ;  Gyudo Lee  ;  Jingam Park  ;  Unchul Shin  ;  Jong Ho Won  ;  Yong Jun Jo  ;  Jin Woo Chang  ;  Sangwoo Lee  ;  Daesung Yoon  ;  Chul-Woo Kim  ;  Jongbum Seo 
Citation
 Scientific Reports, Vol.5 : 9846-9846, 2015 
Journal Title
 Scientific Reports 
ISSN
 2045-2322 
Issue Date
2015
Abstract
Supercoiling DNA (folding DNA into a more compact molecule) from open circular forms requires significant bending energy. The double helix is coiled into a higher order helix form; thus it occupies a smaller footprint. Compact packing of DNA is essential to improve the efficiency of gene delivery, which has broad implications in biology and pharmaceutical research. Here we show that low-intensity pulsed ultrasound can pack open circular DNA into supercoil form. Plasmid DNA subjected to 5.4 mW/cm(2) intensity ultrasound showed significant (p-values <0.001) supercoiling compared to DNA without exposure to ultrasound. Radiation force induced from ultrasound and dragging force from the fluid are believed to be the main factors that cause supercoiling. This study provides the first evidence to show that low-intensity ultrasound can directly alter DNA topology. We anticipate our results to be a starting point for improved non-viral gene delivery.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140632
DOI
10.1038/srep09846
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실)
Yonsei Authors
장진우(Chang, Jin Woo)
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