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Alteration in the intrafollicular thiol-redox system in infertile women with endometriosis

Authors
 Young Sik Choi  ;  SiHyun Cho  ;  Seok Kyo Seo  ;  Joo Hyun Park  ;  Seok Hyun Kim  ;  Byung Seok Lee 
Citation
 Reproduction, Vol.149(2) : 155-162, 2015 
Journal Title
 Reproduction 
ISSN
 1470-1626 
Issue Date
2015
Abstract
The aim of this study was to compare intrafollicular biomarkers of thiol-redox system and chronic inflammation in infertile patients with and without endometriosis, and examine correlations between biomarkers and IVF outcomes. The study included 65 patients receiving IVF: 31 patients with endometriosis vs 34 patients without endometriosis. Follicular fluid (FF) was obtained from a single-dominant follicle during oocyte retrieval and stored at -70 °C. Malondialdehyde, superoxide dismutase, glutathione (GSH), glutathione peroxidase 3 (GPX3), thioredoxin (TRX), TRX-binding protein 2 (TBP2), and peroxiredoxin-4 levels were measured in the FF samples by ELISAs as biomarkers of oxidative stress. The inflammatory cytokines interleukin 1 beta (IL1β), IL6, IL8, and tumor-necrosis factor alpha (TNFα) were also measured by ELISAs. GSH levels were significantly lower in the endometriosis group compared with the controls. TBP2 levels were significantly higher in the endometriosis group. IL6, IL8, and TNFα levels were significantly higher in the endometriosis group. The levels of all of the inflammatory cytokines positively correlated with the levels of TRX. GSH levels positively correlated with the number of high-quality embryos. GPX3 and TRX levels negatively correlated with the percentage of mature oocytes. TNFα levels negatively correlated with the cumulative embryo score per embryo. Logistic regression analysis revealed that the number of high-quality embryos was an independent factor predicting clinical pregnancy. In conclusion, there may be an imbalance in the thiol-redox system and increased levels of inflammatory cytokines in the intrafollicular microenvironment of infertile patients with endometriosis, which may affect the qualities of the oocyte and embryo.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140578
Full Text
http://www.reproduction-online.org/content/149/2/155.long
DOI
10.1530/REP-14-0438
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실)
Yonsei Authors
박주현(Park, Joo Hyun)
서석교(Seo, Seok Kyo) ORCID logo https://orcid.org/0000-0003-3404-0484
이병석(Lee, Byung Seok)
조시현(Cho, Si Hyun)
최영식(Choi, Young Sik)
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