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Fracture Healing Effects of Locally-Administered Adipose Tissue-Derived Cells

Authors
 Sheen-Woo Lee  ;  Tae Joo Jeon  ;  Sandip Biswal 
Citation
 Yonsei Medical Journal, Vol.56(4) : 1106-1113, 2015 
Journal Title
 Yonsei Medical Journal 
ISSN
 0513-5796 
Issue Date
2015
Abstract
PURPOSE: Although the applications of adipose tissue-derived cells (ADCs) in regenerative medicine have been investigated, the role of ADCs in fracture healing remains unclear. In this study, we examined the fracture-healing effects and survival of transplanted ADCs using micro-computed tomography (CT) and bioluminescence imaging (BLI). MATERIALS AND METHODS: Luciferase-expressing ADCs were suspended in solubilized basement membrane preparation (SBMP) and xenografted on defects in the right femur of nude mice (n=5). SBMP alone was grafted on a defect in the contralateral femur. Serial in vivo micro-CT and BLI were performed for 20 days. Ex vivo BLI images of both femurs were obtained. Differences in the Hounsfield unit (HU), HU(ratio), and luciferase activities were compared using Wilcoxon signed-rank tests and non-parametric longitudinal analyses (p<0.05). RESULTS: In vivo BLI revealed a signal drop on day 2, reconstitution on day 5, and continuous decrement thereafter. Ex vivo BLI revealed residual activity in the ADC-implanted and adjacent areas. No activity was detected in the contralateral femur. The overall increment rate of normalized HUs was higher for ADC-treated femurs than for SBMP-treated femurs. Cell migration to distant injury sites was not detected.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140573
DOI
10.3349/ymj.2015.56.4.1106
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Nuclear Medicine (핵의학교실)
Yonsei Authors
전태주(Jeon, Tae Joo)
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