Cited 16 times in
Brimonidine reduces TGF-beta-induced extracellular matrix synthesis in human Tenon's fibroblasts
DC Field | Value | Language |
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dc.contributor.author | 홍사민 | - |
dc.contributor.author | 김찬윤 | - |
dc.contributor.author | 성공제 | - |
dc.contributor.author | 송유경 | - |
dc.contributor.author | 한승한 | - |
dc.contributor.author | 김강윤 | - |
dc.date.accessioned | 2016-02-04T11:21:39Z | - |
dc.date.available | 2016-02-04T11:21:39Z | - |
dc.date.issued | 2015 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/140252 | - |
dc.description.abstract | BACKGROUND: Brimonidine is a highly selective α2 adrenergic agonist that has been widely used in anti-glaucoma eyedrops. The aim of this study was to investigate its putative anti-fibrotic role in the fibrosis caused by activated Tenon's fibroblasts. METHODS: Primary cultured human Tenon's fibroblasts were exposed to 2.0 ng/mL of transforming growth factor-β1 (TGF-β1) for up to 48 h. In the presence of various concentrations of brimonidine (from 0.0 to 10.0 μM), the expression levels of fibronectin, collagen types I and III, and β-actin were determined by Western immunoblots. The expression of phosphorylated SMAD2/3 (p-SMAD2/3) was then evaluated using immunofluorescence. RESULTS: TGF-β1 significantly increased the synthesis of fibronectin and collagens in human Tenon's fibroblasts; however brimonidine treatment distinctly attenuated the TGF-β1-induced production of extracellular matrix (ECM) proteins. TGF-β1 also changed the cellular morphology to be plump, while brimonidine treatment returned the cells to a spindle shape, similar to control fibroblasts. Regarding p-SMAD2/3, brimonidine treatment did not show any apparent changes in its expression. CONCLUSIONS: Our data revealed that brimonidine reduces TGF-β-induced ECM synthesis in human Tenon's fibroblasts in vitro. This finding implies that topical administration of brimonidine may be helpful in reducing the fibrosis caused by the long-term use of topical anti-glaucoma medications. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | BMC OPHTHALMOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Actins/metabolism | - |
dc.subject.MESH | Adrenergic alpha-2 Receptor Agonists/pharmacology* | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Brimonidine Tartrate/pharmacology* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Collagen Type I/metabolism | - |
dc.subject.MESH | Collagen Type III/metabolism | - |
dc.subject.MESH | Extracellular Matrix Proteins/metabolism* | - |
dc.subject.MESH | Fibroblasts/drug effects* | - |
dc.subject.MESH | Fibroblasts/metabolism | - |
dc.subject.MESH | Fibroblasts/pathology | - |
dc.subject.MESH | Fibronectins | - |
dc.subject.MESH | Fibrosis | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Tenon Capsule/cytology* | - |
dc.subject.MESH | Transforming Growth Factor beta1/pharmacology* | - |
dc.title | Brimonidine reduces TGF-beta-induced extracellular matrix synthesis in human Tenon's fibroblasts | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학) | - |
dc.contributor.googleauthor | Samin Hong | - |
dc.contributor.googleauthor | Sueng-Han Han | - |
dc.contributor.googleauthor | Chan Yun Kim | - |
dc.contributor.googleauthor | Kang Yoon Kim | - |
dc.contributor.googleauthor | Yoo Kyung Song | - |
dc.contributor.googleauthor | Gong Je Seong | - |
dc.identifier.doi | 10.1186/s12886-015-0045-8 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04395 | - |
dc.contributor.localId | A01035 | - |
dc.contributor.localId | A01946 | - |
dc.contributor.localId | A02046 | - |
dc.contributor.localId | A04303 | - |
dc.relation.journalcode | J00370 | - |
dc.identifier.eissn | 1471-2415 | - |
dc.identifier.pmid | 26017119 | - |
dc.subject.keyword | Fibroblast | - |
dc.subject.keyword | Fibrosis | - |
dc.subject.keyword | Brimonidine | - |
dc.subject.keyword | Transforming growth factor-β | - |
dc.subject.keyword | Glaucoma | - |
dc.contributor.alternativeName | Hong, Sa Min | - |
dc.contributor.alternativeName | Kim, Chan Yun | - |
dc.contributor.alternativeName | Seong, Gong Je | - |
dc.contributor.alternativeName | Song, Yoo Kyung | - |
dc.contributor.alternativeName | Han, Seung Han | - |
dc.contributor.affiliatedAuthor | Hong, Sa Min | - |
dc.contributor.affiliatedAuthor | Kim, Chan Yun | - |
dc.contributor.affiliatedAuthor | Seong, Gong Je | - |
dc.contributor.affiliatedAuthor | Song, Yoo Kyung | - |
dc.contributor.affiliatedAuthor | Han, Seung Han | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 15 | - |
dc.citation.startPage | 54 | - |
dc.identifier.bibliographicCitation | BMC OPHTHALMOLOGY, Vol.15 : 54, 2015 | - |
dc.identifier.rimsid | 50379 | - |
dc.type.rims | ART | - |
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