393 717

Cited 16 times in

Brimonidine reduces TGF-beta-induced extracellular matrix synthesis in human Tenon's fibroblasts

DC Field Value Language
dc.contributor.author홍사민-
dc.contributor.author김찬윤-
dc.contributor.author성공제-
dc.contributor.author송유경-
dc.contributor.author한승한-
dc.contributor.author김강윤-
dc.date.accessioned2016-02-04T11:21:39Z-
dc.date.available2016-02-04T11:21:39Z-
dc.date.issued2015-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/140252-
dc.description.abstractBACKGROUND: Brimonidine is a highly selective α2 adrenergic agonist that has been widely used in anti-glaucoma eyedrops. The aim of this study was to investigate its putative anti-fibrotic role in the fibrosis caused by activated Tenon's fibroblasts. METHODS: Primary cultured human Tenon's fibroblasts were exposed to 2.0 ng/mL of transforming growth factor-β1 (TGF-β1) for up to 48 h. In the presence of various concentrations of brimonidine (from 0.0 to 10.0 μM), the expression levels of fibronectin, collagen types I and III, and β-actin were determined by Western immunoblots. The expression of phosphorylated SMAD2/3 (p-SMAD2/3) was then evaluated using immunofluorescence. RESULTS: TGF-β1 significantly increased the synthesis of fibronectin and collagens in human Tenon's fibroblasts; however brimonidine treatment distinctly attenuated the TGF-β1-induced production of extracellular matrix (ECM) proteins. TGF-β1 also changed the cellular morphology to be plump, while brimonidine treatment returned the cells to a spindle shape, similar to control fibroblasts. Regarding p-SMAD2/3, brimonidine treatment did not show any apparent changes in its expression. CONCLUSIONS: Our data revealed that brimonidine reduces TGF-β-induced ECM synthesis in human Tenon's fibroblasts in vitro. This finding implies that topical administration of brimonidine may be helpful in reducing the fibrosis caused by the long-term use of topical anti-glaucoma medications.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.relation.isPartOfBMC OPHTHALMOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHActins/metabolism-
dc.subject.MESHAdrenergic alpha-2 Receptor Agonists/pharmacology*-
dc.subject.MESHBlotting, Western-
dc.subject.MESHBrimonidine Tartrate/pharmacology*-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCollagen Type I/metabolism-
dc.subject.MESHCollagen Type III/metabolism-
dc.subject.MESHExtracellular Matrix Proteins/metabolism*-
dc.subject.MESHFibroblasts/drug effects*-
dc.subject.MESHFibroblasts/metabolism-
dc.subject.MESHFibroblasts/pathology-
dc.subject.MESHFibronectins-
dc.subject.MESHFibrosis-
dc.subject.MESHHumans-
dc.subject.MESHTenon Capsule/cytology*-
dc.subject.MESHTransforming Growth Factor beta1/pharmacology*-
dc.titleBrimonidine reduces TGF-beta-induced extracellular matrix synthesis in human Tenon's fibroblasts-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Ophthalmology (안과학)-
dc.contributor.googleauthorSamin Hong-
dc.contributor.googleauthorSueng-Han Han-
dc.contributor.googleauthorChan Yun Kim-
dc.contributor.googleauthorKang Yoon Kim-
dc.contributor.googleauthorYoo Kyung Song-
dc.contributor.googleauthorGong Je Seong-
dc.identifier.doi10.1186/s12886-015-0045-8-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA04395-
dc.contributor.localIdA01035-
dc.contributor.localIdA01946-
dc.contributor.localIdA02046-
dc.contributor.localIdA04303-
dc.relation.journalcodeJ00370-
dc.identifier.eissn1471-2415-
dc.identifier.pmid26017119-
dc.subject.keywordFibroblast-
dc.subject.keywordFibrosis-
dc.subject.keywordBrimonidine-
dc.subject.keywordTransforming growth factor-β-
dc.subject.keywordGlaucoma-
dc.contributor.alternativeNameHong, Sa Min-
dc.contributor.alternativeNameKim, Chan Yun-
dc.contributor.alternativeNameSeong, Gong Je-
dc.contributor.alternativeNameSong, Yoo Kyung-
dc.contributor.alternativeNameHan, Seung Han-
dc.contributor.affiliatedAuthorHong, Sa Min-
dc.contributor.affiliatedAuthorKim, Chan Yun-
dc.contributor.affiliatedAuthorSeong, Gong Je-
dc.contributor.affiliatedAuthorSong, Yoo Kyung-
dc.contributor.affiliatedAuthorHan, Seung Han-
dc.rights.accessRightsfree-
dc.citation.volume15-
dc.citation.startPage54-
dc.identifier.bibliographicCitationBMC OPHTHALMOLOGY, Vol.15 : 54, 2015-
dc.identifier.rimsid50379-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.