Cited 13 times in
IgE reactivity to carbohydrate moieties of glycoproteins in wheat allergy
DC Field | Value | Language |
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dc.contributor.author | 김경원 | - |
dc.contributor.author | 김규언 | - |
dc.contributor.author | 김미나 | - |
dc.contributor.author | 김윤희 | - |
dc.contributor.author | 손명현 | - |
dc.contributor.author | 홍정연 | - |
dc.date.accessioned | 2016-02-04T11:19:57Z | - |
dc.date.available | 2016-02-04T11:19:57Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1088-5412 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/140188 | - |
dc.description.abstract | Carbohydrate moieties of different glycoproteins, such as cross-reactive carbohydrate determinants (CCDs) and galactose α-1,3-galactose, can induce IgE reactivity with varied clinical significance. In this study, the possible participation of glycan from wheat gliadin, with respect to its IgE-binding capacity, was investigated in children with food allergies to wheat. Total IgE and wheat-specific IgE quantification, documentation of history, and/or oral food challenge (OFC) were performed for 52 children. Subjects with positive wheat-specific IgE were characterized as the symptomatic group, never-exposed group, or asymptomatic group. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and glycan detection in gliadin were performed. IgE binding to gliadin and deglycosylated gliadin was measured by immunoblotting and ELISA. Gliadin-specific IgE was detected and correlated with wheat-specific IgE in the symptomatic, never-exposed, and asymptomatic groups. The glycan range overlapped significantly with the gliadin range. Deglycosylation of gliadin reduced the allergenicity of gliadin. In gliadin, the allergenicity of the glycan portion was greater in the symptomatic group than in the never-exposed and asymptomatic groups. We conclude that N-glycan in gliadin might exhibit allergenicity as a possible carbohydrate epitope in wheat allergy in children. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 192~199 | - |
dc.relation.isPartOf | ALLERGY AND ASTHMA PROCEEDINGS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Allergens/immunology* | - |
dc.subject.MESH | Carbohydrates/immunology* | - |
dc.subject.MESH | Case-Control Studies | - |
dc.subject.MESH | Child | - |
dc.subject.MESH | Child, Preschool | - |
dc.subject.MESH | Cytokines/blood | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gliadin/chemistry | - |
dc.subject.MESH | Gliadin/immunology | - |
dc.subject.MESH | Glycoproteins/chemistry | - |
dc.subject.MESH | Glycoproteins/immunology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoglobulin E/blood | - |
dc.subject.MESH | Immunoglobulin E/immunology* | - |
dc.subject.MESH | Infant | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Protein Binding/immunology | - |
dc.subject.MESH | Triticum/adverse effects* | - |
dc.subject.MESH | Wheat Hypersensitivity/blood | - |
dc.subject.MESH | Wheat Hypersensitivity/diagnosis | - |
dc.subject.MESH | Wheat Hypersensitivity/immunology* | - |
dc.title | IgE reactivity to carbohydrate moieties of glycoproteins in wheat allergy | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | Tae Won Song | - |
dc.contributor.googleauthor | Jung Yeon Hong | - |
dc.contributor.googleauthor | Kyung Eun Lee | - |
dc.contributor.googleauthor | Mi Na Kim | - |
dc.contributor.googleauthor | Yoon Hee Kim | - |
dc.contributor.googleauthor | Soo-Young Lee | - |
dc.contributor.googleauthor | Kyung Won Kim | - |
dc.contributor.googleauthor | Myung Hyun Sohn | - |
dc.contributor.googleauthor | Kyu-Earn Kim | - |
dc.identifier.doi | 10.2500/aap.2015.36.3815 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00303 | - |
dc.contributor.localId | A00327 | - |
dc.contributor.localId | A00441 | - |
dc.contributor.localId | A00799 | - |
dc.contributor.localId | A01967 | - |
dc.contributor.localId | A04431 | - |
dc.relation.journalcode | J00063 | - |
dc.identifier.eissn | 1539-6304 | - |
dc.identifier.pmid | 25976436 | - |
dc.subject.keyword | Carbohydrate epitope | - |
dc.subject.keyword | cross-reactive carbohydrate determinants | - |
dc.subject.keyword | deglycosylation | - |
dc.subject.keyword | food allergy | - |
dc.subject.keyword | gliadin | - |
dc.subject.keyword | glycan | - |
dc.subject.keyword | glycoprotein | - |
dc.subject.keyword | immunoglobulin E reactivity | - |
dc.subject.keyword | oral food challenge | - |
dc.subject.keyword | wheat allergy | - |
dc.contributor.alternativeName | Kim, Kyung Won | - |
dc.contributor.alternativeName | Kim, Kyu Earn | - |
dc.contributor.alternativeName | Kim, Mina | - |
dc.contributor.alternativeName | Kim, Yoon Hee | - |
dc.contributor.alternativeName | Son, Myung Hyun | - |
dc.contributor.alternativeName | Hong, Jung Yeon | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Won | - |
dc.contributor.affiliatedAuthor | Kim, Kyu Earn | - |
dc.contributor.affiliatedAuthor | Kim, Mina | - |
dc.contributor.affiliatedAuthor | Kim, Yoon Hee | - |
dc.contributor.affiliatedAuthor | Son, Myung Hyun | - |
dc.contributor.affiliatedAuthor | Hong, Jung Yeon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 36 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 192 | - |
dc.citation.endPage | 199 | - |
dc.identifier.bibliographicCitation | ALLERGY AND ASTHMA PROCEEDINGS, Vol.36(3) : 192-199, 2015 | - |
dc.identifier.rimsid | 53954 | - |
dc.type.rims | ART | - |
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