Cited 5 times in
Ca(2+) is a Regulator of the WNK/OSR1/NKCC Pathway in a Human Salivary Gland Cell Line
DC Field | Value | Language |
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dc.contributor.author | 박순홍 | - |
dc.contributor.author | 신동민 | - |
dc.contributor.author | 지혜원 | - |
dc.contributor.author | 최종훈 | - |
dc.date.accessioned | 2016-02-04T11:15:52Z | - |
dc.date.available | 2016-02-04T11:15:52Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1226-4512 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/140035 | - |
dc.description.abstract | Wnk kinase maintains cell volume, regulating various transporters such as sodium-chloride cotransporter, potassium-chloride cotransporter, and sodium-potassium-chloride cotransporter 1 (NKCC1) through the phosphorylation of oxidative stress responsive kinase 1 (OSR1) and STE20/SPS1-related proline/alanine-rich kinase (SPAK). However, the activating mechanism of Wnk kinase in specific tissues and specific conditions is broadly unclear. In the present study, we used a human salivary gland (HSG) cell line as a model and showed that Ca(2+) may have a role in regulating Wnk kinase in the HSG cell line. Through this study, we found that the HSG cell line expressed molecules participating in the WNK-OSR1-NKCC pathway, such as Wnk1, Wnk4, OSR1, SPAK, and NKCC1. The HSG cell line showed an intracellular Ca(2+) concentration ([Ca(2+)]i) increase in response to hypotonic stimulation, and the response was synchronized with the phosphorylation of OSR1. Interestingly, when we inhibited the hypotonically induced [Ca(2+)]i increase with nonspecific Ca(2+) channel blockers such as 2-aminoethoxydiphenyl borate, gadolinium, and lanthanum, the phosphorylated OSR1 level was also diminished. Moreover, a cyclopiazonic acid-induced passive [Ca(2+)]i elevation was evoked by the phosphorylation of OSR1, and the amount of phosphorylated OSR1 decreased when the cells were treated with BAPTA, a Ca(2+) chelator. Finally, through that process, NKCC1 activity also decreased to maintain the cell volume in the HSG cell line. These results indicate that Ca(2+) may regulate the WNK-OSR1 pathway and NKCC1 activity in the HSG cell line. This is the first demonstration that indicates upstream Ca(2+) regulation of the WNK-OSR1 pathway in intact cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 249~255 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Ca(2+) is a Regulator of the WNK/OSR1/NKCC Pathway in a Human Salivary Gland Cell Line | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Soonhong Park | - |
dc.contributor.googleauthor | Sang Kyun Ku | - |
dc.contributor.googleauthor | Hye Won Ji | - |
dc.contributor.googleauthor | Jong Hoon Choi | - |
dc.contributor.googleauthor | Dong Min Shin | - |
dc.identifier.doi | 10.4196/kjpp.2015.19.3.249 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01547 | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A03974 | - |
dc.contributor.localId | A04188 | - |
dc.relation.journalcode | J02104 | - |
dc.identifier.eissn | 2093-3827 | - |
dc.identifier.pmid | 25954130 | - |
dc.subject.keyword | Ca2+ signaling | - |
dc.subject.keyword | NKCC | - |
dc.subject.keyword | OSR1 | - |
dc.subject.keyword | Salivary gland | - |
dc.subject.keyword | WNK | - |
dc.contributor.alternativeName | Park, Soon Hong | - |
dc.contributor.alternativeName | Shin, Dong Min | - |
dc.contributor.alternativeName | Ji, Hye Won | - |
dc.contributor.alternativeName | Choi, Jong Hoon | - |
dc.contributor.affiliatedAuthor | Park, Soon Hong | - |
dc.contributor.affiliatedAuthor | Shin, Dong Min | - |
dc.contributor.affiliatedAuthor | Ji, Hye Won | - |
dc.contributor.affiliatedAuthor | Choi, Jong Hoon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 19 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 249 | - |
dc.citation.endPage | 255 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, Vol.19(3) : 249-255, 2015 | - |
dc.identifier.rimsid | 45586 | - |
dc.type.rims | ART | - |
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