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Differential expression of cancer-associated fibroblast-related proteins according to molecular subtype and stromal histology in breast cancer

Authors
 Sung Yeon Park  ;  Hye Min Kim  ;  Ja Seung Koo 
Citation
 Breast Cancer Research and Treatment, Vol.149(3) : 727-741, 2015 
Journal Title
 Breast Cancer Research and Treatment 
ISSN
 0167-6806 
Issue Date
2015
MeSH
Adult ; Aged ; Biomarkers, Tumor/biosynthesis* ; Biomarkers, Tumor/genetics ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Immunohistochemistry/methods ; Kaplan-Meier Estimate ; Middle Aged ; Molecular Targeted Therapy ; Neoplasm Proteins/biosynthesis* ; Prognosis* ; Receptor, ErbB-2/genetics ; Tissue Array Analysis ; Triple Negative Breast Neoplasms/genetics* ; Triple Negative Breast Neoplasms/pathology
Keywords
Breast cancer ; Cancer-associated fibroblast ; Molecular subtype ; Tumor stroma
Abstract
The purpose of this study aimed to investigate the clinicopathologic characteristics of breast cancer according to its cancer-associated fibroblast (CAF) phenotype. Immunohistochemistry staining of estrogen receptor, progesterone receptor, human epidermal growth factor receptor 2 (HER-2), Ki-67, podoplanin, prolyl 4-hydroxylase, fibroblast activation protein alpha (FAPα), S100A4, platelet-derived growth factor receptor alpha (PDGFRα), PDGFRβ, and chondroitin sulfate proteoglycan (NG2) was performed on tissue microarray consisting of 642 breast cancer cases. Samples were categorized into luminal A, luminal B, HER-2, or triple-negative breast cancer (TNBC) according to immunohistochemical results, whereas tumor stroma was classified into desmoplastic, sclerotic, normal-like, or inflammatory type based on histological findings. Expression of CAF-related proteins in the stroma differed depending on breast cancer molecular subtypes. All CAF-related protein expression was high (p < 0.05) in HER-2 type, whereas in luminal A, the expression of FAPα, PDGFα, PDGFβ, and NG2 was low, and in TNBC, the expression of podoplanin, prolyl 4-hydroxylase, and S100A4 was low. In the stromal component, CAF-related protein expression differed according to stromal phenotype (p < 0.001). The desmoplastic type showed high expression of podoplanin, prolyl 4-hydroxylase, S100A4, PDGFRα, and PDGFRβ, whereas the sclerotic type exhibited low expression of FAPα, PDGFα, PDGFβ, and NG2. The inflammatory type had high expression of FAPα and NG2 with low podoplanin, while normal-like type showed low expression of prolyl 4-hydroxylase and S100A4. Our results suggested that differential CAF-related protein expression depended on the molecular subtypes and stromal histologic features of breast cancer, indicating that in the future, this system could potentially use these markers for prognosis prediction and targeted therapy of breast cancer.
Full Text
http://link.springer.com/article/10.1007%2Fs10549-015-3291-9
DOI
10.1007/s10549-015-3291-9
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Koo, Ja Seung(구자승) ORCID logo https://orcid.org/0000-0003-4546-4709
Kim, Hye Min(김혜민) ORCID logo https://orcid.org/0000-0002-2899-9480
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139428
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