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RUNX3 expression is associated with sensitivity to pheophorbide a-based photodynamic therapy in keloids

 Zhenlong Zheng  ;  Lianhua Zhu  ;  Xianglan Zhang  ;  Lianhua Li  ;  Sook Moon  ;  Mi Ryung Roh  ;  Zhehu Jin 
 LASERS IN MEDICAL SCIENCE, Vol.30(1) : 67-75, 2015 
Journal Title
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Adolescent ; Adult ; Aged ; Aged, 80 and over ; Apoptosis/drug effects ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Child ; Chlorophyll/analogs & derivatives* ; Chlorophyll/pharmacology ; Chlorophyll/therapeutic use ; Collagen Type I/metabolism ; Core Binding Factor Alpha 3 Subunit/metabolism* ; Down-Regulation/drug effects ; Female ; Fibroblasts/drug effects ; Fibroblasts/metabolism ; Fibroblasts/pathology ; Humans ; Keloid/drug therapy* ; Keloid/metabolism ; Keloid/pathology ; Male ; Middle Aged ; Photochemotherapy* ; Proliferating Cell Nuclear Antigen/metabolism ; Reactive Oxygen Species/metabolism ; Skin/drug effects ; Skin/pathology ; Young Adult
RUNX3 ; Pheophorbide a-based photodynamic therapy ; Keloid
Runt-related transcription factor 3 (RUNX3) has recently been reported to be a possible predictor of sensitivity of cancer cells for photodynamic therapy (PDT), a promising therapeutic modality for keloids. In this study, we aimed to elucidate the implications of RUNX3 for keloid pathogenesis and sensitivity to pheophorbide a-based PDT (Pa-PDT). RUNX3 and proliferating cell nuclear antigen (PCNA) expression were examined in 6 normal skin samples and 32 keloid tissue samples by immunohistochemistry. We found that RUNX3 expression was detected more often in keloid tissues than in dermis of normal skin. In keloid tissues, RUNX3 expression was significantly increased in patients presenting with symptoms of pain or pruritus, and was also significantly related to PCNA expression. The therapeutic effect of Pa-PDT was comparatively investigated in keloid fibroblasts (KFs) with and without RUNX3 expression. Significant differences were found after Pa-PDT between KFs with and without RUNX3 expression in cell viability, proliferative ability, type I collagen expression, generation of reactive oxygen species (ROS), and apoptotic cell death. In addition, RUNX3 expression was significantly decreased after Pa-PDT in KFs, and KFs with downregulation of RUNX3 showed significantly increased cell viability after Pa-PDT. Pa-PDT may be a potential therapeutic modality for keloids, and RUNX3, as a possible contributor to keloid pathogenesis, may improve sensitivity to Pa-PDT in KFs.
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1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
Yonsei Authors
Roh, Mi Ryung(노미령) ORCID logo https://orcid.org/0000-0002-6285-2490
Zhang, Xiang Lan(장향란)
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