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Continuous enrichment of circulating tumor cells using a microfluidic lateral flow filtration chip

Authors
 Sung-Woo Lee  ;  Kyung-A. Hyun  ;  Seung-Il Kim  ;  Ji-Yoon Kang  ;  Hyo-Il Jung 
Citation
 JOURNAL OF CHROMATOGRAPHY A, Vol.1377 : 100-105, 2015 
Journal Title
 JOURNAL OF CHROMATOGRAPHY A 
ISSN
 0021-9673 
Issue Date
2015
MeSH
Cell Line, Tumor ; Cell Separation/methods ; Filtration/instrumentation ; Humans ; Microfluidics* ; Neoplastic Cells, Circulating/metabolism
Keywords
Circulating tumor cells(CTCs) ; Enrichment ; Lateral flow filtration ; Microfluidics
Abstract
The isolation and characterization of circulating tumor cells (CTC) is of great importance in cancer diagnosis and prognosis. Highly sensitive detection of CTCs can be very difficult because they are extremely rare (i.e., 1-5 CTCs per 10(9) erythrocytes) in blood. Recently, various devices have been developed that exploit biochemical (affinity-based) and physical (size or density) methods. Antibody-based isolation has its own limitations, as the expression level of the epitopes for an antibody varies due to the heterogeneity of cancer cells. Harsh conditions associated with physical methods can cause the deformation and damage of CTCs during the isolation process. Here, we propose a microfluidic lateral flow filtration (μ-LaFF) chip in which lateral flow was combined with vertical flow into the filter to capture the CTCs gently. The CTCs experienced weak shear flow owing to the lateral flow and traveled alongside the filter channel until finally being captured. The vertical flow in the filter held the captured cells tightly and served as an exit for uncaptured hematological cells (white and red blood cells). From our μ-LaFF chip we obtained a high capture efficiency (95%) and purity (99%), minimizing any damage to the CTCs. Our μ-LaFF technology is expected to be useful in the diagnosis and prognosis of various cancers.
Full Text
http://www.sciencedirect.com/science/article/pii/S0021967314019438
DOI
10.1016/j.chroma.2014.12.037
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seung Il(김승일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139236
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