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Expression of Lymphangiogenic Markers in Rejected Human Corneal Buttons after Penetrating Keratoplasty

DC Field Value Language
dc.contributor.author김응권-
dc.contributor.author이형근-
dc.date.accessioned2016-02-04T10:52:28Z-
dc.date.available2016-02-04T10:52:28Z-
dc.date.issued2015-
dc.identifier.issn0271-3683-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/139169-
dc.description.abstractPURPOSE: To investigate the extent and distribution of lymphangiogenesis in the rejected corneal graft, we determined the expression of several lymphangiogenic markers in rejected human corneal buttons. MATERIAL AND METHODS: Thirty-four corneal buttons were obtained from patients who underwent re-keratoplasty for graft rejection after penetrating keratoplasty. All corneas showed signs of rejection, such as, sudden mutton-fat keratic precipitates (KPs) or lines before re-keratoplasty. The corneas were halved, and one half was used for immunostaining and the other half was used for RT-PCR. Expression of vascular endothelial growth factor (VEGF)-A, VEGF-C, VEGF-D, VEGFR-2, VEGFR-3, LYVE-1 and podoplanin were measured as lymphangiogenic markers. Four non-operated normal corneas were used as controls. RESULTS: Numerous podoplanin positive cells were found in the anterior and posterior stroma. However, LYVE-1 positive mature lymphatics were found only in herpetic keratitis (HK)-induced graft rejection, and not in pseudophakic bullous keratopathy (PBK). RT-PCR showed that levels of VEGF-A, VEGF-C, VEGFR-2, and VEGFR-3 mRNAs were elevated in rejected corneal buttons versus the non-operated control corneas. Based upon the pre-keratoplasty pathologic conditions, HK cases showed higher levels of VEGF-A and VEGFR-2 than PBK. The mRNA ratios (keratoplastic cornea/normal cornea) for VEGF-A and VEGFR-2 were 8.9 and 5.8, respectively. CONCLUSIONS: The results suggested that the VEGF-A and the VEGFR-2 may be a more important pathway for lymphangiogenesis in rejected corneal grafts than the VEGFR-3. In addition, organized lymphangiogenesis is more prominent in HK than PBK.-
dc.description.statementOfResponsibilityopen-
dc.format.extent902~912-
dc.relation.isPartOfCURRENT EYE RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHAged-
dc.subject.MESHBiomarkers/metabolism-
dc.subject.MESHCornea/metabolism*-
dc.subject.MESHCornea/pathology-
dc.subject.MESHFemale-
dc.subject.MESHGene Expression Regulation*-
dc.subject.MESHGraft Rejection/genetics*-
dc.subject.MESHGraft Rejection/metabolism-
dc.subject.MESHGraft Rejection/pathology-
dc.subject.MESHHumans-
dc.subject.MESHKeratoplasty, Penetrating/adverse effects*-
dc.subject.MESHLymphangiogenesis/genetics*-
dc.subject.MESHMale-
dc.subject.MESHMicroscopy, Confocal-
dc.subject.MESHMiddle Aged-
dc.subject.MESHRNA/genetics*-
dc.subject.MESHReal-Time Polymerase Chain Reaction-
dc.subject.MESHVascular Endothelial Growth Factors/biosynthesis-
dc.subject.MESHVascular Endothelial Growth Factors/genetics*-
dc.subject.MESHYoung Adult-
dc.titleExpression of Lymphangiogenic Markers in Rejected Human Corneal Buttons after Penetrating Keratoplasty-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Ophthalmology (안과학)-
dc.contributor.googleauthorYuri Seo-
dc.contributor.googleauthorMee Kum Kim-
dc.contributor.googleauthorJoon H. Lee-
dc.contributor.googleauthorEun-Ju Chang-
dc.contributor.googleauthorEung Kweon Kim-
dc.contributor.googleauthorHyung Keun Lee-
dc.identifier.doi10.3109/02713683.2014.969809-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00831-
dc.contributor.localIdA03303-
dc.relation.journalcodeJ00665-
dc.identifier.eissn1460-2202-
dc.identifier.pmid25330436-
dc.identifier.urlhttp://www.tandfonline.com/doi/full/10.3109/02713683.2014.969809-
dc.subject.keywordLymphangiogenesis-
dc.subject.keywordVEGF-
dc.subject.keywordVEGFR-
dc.subject.keywordpenetrating keratoplasty-
dc.subject.keywordrejection-
dc.contributor.alternativeNameKim, Eung Kweon-
dc.contributor.alternativeNameLee, Hyung Keun-
dc.contributor.affiliatedAuthorKim, Eung Kweon-
dc.contributor.affiliatedAuthorLee, Hyung Keun-
dc.rights.accessRightsnot free-
dc.citation.volume40-
dc.citation.number9-
dc.citation.startPage902-
dc.citation.endPage912-
dc.identifier.bibliographicCitationCURRENT EYE RESEARCH, Vol.40(9) : 902-912, 2015-
dc.identifier.rimsid43807-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers

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