Cited 3 times in
Expression of Lymphangiogenic Markers in Rejected Human Corneal Buttons after Penetrating Keratoplasty
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김응권 | - |
dc.contributor.author | 이형근 | - |
dc.date.accessioned | 2016-02-04T10:52:28Z | - |
dc.date.available | 2016-02-04T10:52:28Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0271-3683 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/139169 | - |
dc.description.abstract | PURPOSE: To investigate the extent and distribution of lymphangiogenesis in the rejected corneal graft, we determined the expression of several lymphangiogenic markers in rejected human corneal buttons. MATERIAL AND METHODS: Thirty-four corneal buttons were obtained from patients who underwent re-keratoplasty for graft rejection after penetrating keratoplasty. All corneas showed signs of rejection, such as, sudden mutton-fat keratic precipitates (KPs) or lines before re-keratoplasty. The corneas were halved, and one half was used for immunostaining and the other half was used for RT-PCR. Expression of vascular endothelial growth factor (VEGF)-A, VEGF-C, VEGF-D, VEGFR-2, VEGFR-3, LYVE-1 and podoplanin were measured as lymphangiogenic markers. Four non-operated normal corneas were used as controls. RESULTS: Numerous podoplanin positive cells were found in the anterior and posterior stroma. However, LYVE-1 positive mature lymphatics were found only in herpetic keratitis (HK)-induced graft rejection, and not in pseudophakic bullous keratopathy (PBK). RT-PCR showed that levels of VEGF-A, VEGF-C, VEGFR-2, and VEGFR-3 mRNAs were elevated in rejected corneal buttons versus the non-operated control corneas. Based upon the pre-keratoplasty pathologic conditions, HK cases showed higher levels of VEGF-A and VEGFR-2 than PBK. The mRNA ratios (keratoplastic cornea/normal cornea) for VEGF-A and VEGFR-2 were 8.9 and 5.8, respectively. CONCLUSIONS: The results suggested that the VEGF-A and the VEGFR-2 may be a more important pathway for lymphangiogenesis in rejected corneal grafts than the VEGFR-3. In addition, organized lymphangiogenesis is more prominent in HK than PBK. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 902~912 | - |
dc.relation.isPartOf | CURRENT EYE RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Biomarkers/metabolism | - |
dc.subject.MESH | Cornea/metabolism* | - |
dc.subject.MESH | Cornea/pathology | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gene Expression Regulation* | - |
dc.subject.MESH | Graft Rejection/genetics* | - |
dc.subject.MESH | Graft Rejection/metabolism | - |
dc.subject.MESH | Graft Rejection/pathology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Keratoplasty, Penetrating/adverse effects* | - |
dc.subject.MESH | Lymphangiogenesis/genetics* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Microscopy, Confocal | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | RNA/genetics* | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction | - |
dc.subject.MESH | Vascular Endothelial Growth Factors/biosynthesis | - |
dc.subject.MESH | Vascular Endothelial Growth Factors/genetics* | - |
dc.subject.MESH | Young Adult | - |
dc.title | Expression of Lymphangiogenic Markers in Rejected Human Corneal Buttons after Penetrating Keratoplasty | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학) | - |
dc.contributor.googleauthor | Yuri Seo | - |
dc.contributor.googleauthor | Mee Kum Kim | - |
dc.contributor.googleauthor | Joon H. Lee | - |
dc.contributor.googleauthor | Eun-Ju Chang | - |
dc.contributor.googleauthor | Eung Kweon Kim | - |
dc.contributor.googleauthor | Hyung Keun Lee | - |
dc.identifier.doi | 10.3109/02713683.2014.969809 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00831 | - |
dc.contributor.localId | A03303 | - |
dc.relation.journalcode | J00665 | - |
dc.identifier.eissn | 1460-2202 | - |
dc.identifier.pmid | 25330436 | - |
dc.identifier.url | http://www.tandfonline.com/doi/full/10.3109/02713683.2014.969809 | - |
dc.subject.keyword | Lymphangiogenesis | - |
dc.subject.keyword | VEGF | - |
dc.subject.keyword | VEGFR | - |
dc.subject.keyword | penetrating keratoplasty | - |
dc.subject.keyword | rejection | - |
dc.contributor.alternativeName | Kim, Eung Kweon | - |
dc.contributor.alternativeName | Lee, Hyung Keun | - |
dc.contributor.affiliatedAuthor | Kim, Eung Kweon | - |
dc.contributor.affiliatedAuthor | Lee, Hyung Keun | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 40 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 902 | - |
dc.citation.endPage | 912 | - |
dc.identifier.bibliographicCitation | CURRENT EYE RESEARCH, Vol.40(9) : 902-912, 2015 | - |
dc.identifier.rimsid | 43807 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.