0 462

Cited 11 times in

Local non-viral gene delivery of apoptin delays the onset of paresis in an experimental model of intramedullary spinal cord tumor.

Authors
 W A Pennant  ;  S An  ;  S J Gwak  ;  S Choi  ;  D T Banh  ;  A B L Nguyen  ;  H Y Song  ;  Y Ha  ;  J S Park 
Citation
 SPINAL CORD, Vol.52(1) : 3-8, 2014 
Journal Title
SPINAL CORD
ISSN
 1362-4393 
Issue Date
2014
MeSH
Animals ; Capsid Proteins/genetics* ; Capsid Proteins/therapeutic use ; Disease Models, Animal ; Gene Transfer Techniques* ; Genetic Therapy/methods* ; Male ; Paresis/etiology ; Paresis/prevention & control* ; Rats ; Rats, Sprague-Dawley ; Spinal Cord Neoplasms/complications*
Abstract
OBJECTIVE: The objective of this study is to evaluate the safety and efficacy of a tumor-specific apoptosis-inducing gene, apoptin, as delivered by the non-viral carrier, PAM-RG4, in an animal model of spinal cord tumor.
METHODS: Male Sprague-Dawley rats were given a 2.5-μl intramedullary injection of C6 glioma (100,000) cells and randomized into three groups (day 0). On day 5, animals received a 7.5-μl intramedullary injection of Dulbecco's modified Eagle's medium (Group 1; n=7), PAM-RG4/control gene polyplex (Group 2; n=7), or PAM-RG4/apoptin gene polyplex (Group 3; n=8). Hindlimb functional strength was assessed every other day for the duration of the study. The spinal cords of killed animals were collected and hematoxylin-eosin stained.
RESULTS: Following treatment, animals that received apoptin had significantly higher mean functional hindlimb scores than those of sham control animals, showing a level of preserved hindlimb function throughout the study. In addition, Group 1 (sham control) and Group 2 (control gene) animals had median survival scores lower than those of animals receiving apoptin. Histopathological analysis showed marked retardation of tumor progression in apoptin-treated animals compared with sham controls.
CONCLUSION: Our study suggests that apoptin is safe for use in the mammalian spinal cord as well as effective in slowing the progression of tumor growth in the spinal cord. The significant slowing of tumor progression, as manifested by the preserved hindlimb function, coupled with the reduction in tumor volume, shows local non-viral delivery of apoptin could serve as an emerging therapy for the treatment of intramedullary spinal cord tumors.
Full Text
http://www.nature.com/sc/journal/v52/n1/full/sc2013106a.html
DOI
10.1038/sc.2013.106
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Ha, Yoon(하윤)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139062
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links