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Molecular mechanism of local drug delivery with Paclitaxel-eluting membranes in biliary and pancreatic cancer: new application for an old drug

Authors
 Sookhee Bang  ;  Sung Ill Jang  ;  Dong Ki Lee  ;  Don Haeng Lee  ;  Sugeun Yang  ;  Kun Na  ;  Eun Ae Jo  ;  Chang Woo Lee  ;  Soo Jin Oh  ;  Jieun Yun  ;  Yi-Yong Baek  ;  Su Yeon Lee 
Citation
 Gastroenterology Research and Practice, Vol.2015(2015), 2014 
Journal Title
 Gastroenterology Research and Practice 
ISSN
 1687-6121 
Issue Date
2014
Abstract
Implantation of self-expanding metal stents (SEMS) is palliation for patients suffering from inoperable malignant obstructions associated with biliary and pancreatic cancers. Chemotherapeutic agent-eluting stents have been developed because SEMS are susceptible to occlusion by tumor in-growth. We reported recently that paclitaxel-eluting SEMS provide enhanced local drug delivery in an animal model. However, little is known about the molecular mechanisms by which paclitaxel-eluting stents attenuate tumor growth. We investigated the signal transduction pathways underlying the antiproliferative effects of a paclitaxel-eluting membrane (PEM) implanted in pancreatic/cholangiocarcinoma tumor bearing nude mice. Molecular and cellular alterations were analyzed in the PEM-implanted pancreatic/cholangiocarcinoma xenograft tumors by Western blot, immunoprecipitation, and immunofluorescence. The quantities of paclitaxel released into the tumor and plasma were determined by liquid chromatography-tandem mass spectroscopy. Paclitaxel from the PEM and its diffusion into the tumor inhibited angiogenesis, which involved suppression of mammalian target of rapamycin (mTOR) through regulation of hypoxia inducible factor (HIF-1) and increased apoptosis. Moreover, implantation of the PEM inhibited tumor-stromal interaction-related expression of proteins such as CD44, SPARC, matrix metalloproteinase-2, and vimentin. Local delivery of paclitaxel from a PEM inhibited growth of pancreatic/cholangiocarcinoma tumors in nude mice by suppressing angiogenesis via the mTOR and inducing apoptosis signal pathway.
Files in This Item:
T201504984.pdf Download
DOI
10.1155/2015/568981
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
이동기(Lee, Dong Ki) ORCID logo https://orcid.org/0000-0002-0048-9112
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139017
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