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Evaluation of a quantitative RT-PCR assay to detect HER2 mRNA overexpression for diagnosis and selection of trastuzumab therapy in breast cancer tissue samples.

Authors
 Hye young Wang  ;  Sunghyun Kim  ;  Sangjung Park  ;  Seungil Kim  ;  Dongju Jung  ;  Kwang Hwa Park  ;  Hyeyoung Lee 
Citation
 EXPERIMENTAL AND MOLECULAR PATHOLOGY, Vol.97(3) : 368-374, 2014 
Journal Title
 EXPERIMENTAL AND MOLECULAR PATHOLOGY 
ISSN
 0014-4800 
Issue Date
2014
MeSH
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antibodies, Monoclonal, Humanized/therapeutic use ; Antineoplastic Agents/therapeutic use ; Breast Neoplasms/diagnosis* ; Breast Neoplasms/drug therapy ; Female ; Genes, erbB-2 ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Middle Aged ; Molecular Targeted Therapy/methods* ; Polymerase Chain Reaction/methods* ; RNA, Messenger/analysis* ; Receptor, ErbB-2/analysis* ; Sensitivity and Specificity ; Trastuzumab ; Young Adult
Keywords
Breast cancer ; FFPE tissue ; HER2 ; Herceptin (trastuzumab) ; Molecular diagnosis ; RT-qPCR
Abstract
Breast cancer patients who have a positive result for HER2 overexpression are commonly treated with Herceptin, a HER2-targeted therapy. In the present study, the BrightGen HER2 RT-qDx (Syantra, Calgary, Canada), which is based on a one-tube nested RT-qPCR method that detects HER2 mRNA overexpression, was clinically evaluated in a total of 237 formalin-fixed paraffin-embedded (FFPE) tissue samples from breast cancer patients. Among the 38 HER2 positive samples, which were determined via IHC/FISH methods, 13 samples out of 16 (81.3%) that were IHC2+/FISH+ and 22 samples out of 22 (100%) that were IHC3+ have been decided positive for HER2 expression via the RT-qPCR method. The true positivity and false positivity results for the RT-qPCR were 92% (35/38) and 2% (1/65), respectively. The concordance between RT-qPCR and IHC results and RT-qPCR and IHC/FISH was 87.2% and 92.1%, respectively. Conclusively, the BrightGen HER2 RT-qDx may be a reliable and convenient method that can supplement traditional IHC and FISH methods for efficient use of trastuzumab.
Full Text
http://www.sciencedirect.com/science/article/pii/S001448001400152X
DOI
10.1016/j.yexmp.2014.09.011
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seung Il(김승일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/138605
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