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Enrichment of circulating tumor cells using a centrifugal affinity plate system

Authors
 Sung Woo Lee  ;  Kyung A. Hyun  ;  Seung Il Kim  ;  Ji Yoon Kang  ;  Hyo Il Jung 
Citation
 JOURNAL OF CHROMATOGRAPHY A, Vol.1373 : 25-30, 2014 
Journal Title
 JOURNAL OF CHROMATOGRAPHY A 
ISSN
 0021-9673 
Issue Date
2014
MeSH
Cell Line, Tumor ; Cell Survival ; Centrifugation/instrumentation* ; Centrifugation/methods ; Humans ; Neoplastic Cells, Circulating*/pathology
Keywords
Centrifugal force ; Circulating tumor cell (CTC) ; Epithelial adhesion molecule (EpCAM) ; High throughput
Abstract
Circulating tumor cells (CTCs) are defined as cells that have detached from a primary tumor and are circulating in the bloodstream. Their isolation and quantification is of great value for cancer prognoses and drug testing. Here, the development of a centrifugal affinity plate (CAP) system is described, in which centrifugal force and antibody-based capture are exploited to enrich CTCs on one plate and hematological cells on the other. The CAP is rotated to exert centrifugal force on the cells in a blood sample, quickly transporting them to the anti-epithelial adhesion molecule (EpCAM)-coated and anti-CD45-coated surface of the CAP to shorten the reaction time and increase the adhesion force between the tumor and blood cells and each antibody. The effect of a rotating process on cell capture was investigated, and the capture efficiency was demonstrated using blood samples from healthy donors spiked with human non-small cell lung cancer (NCI-H1650) and breast cancer (MCF-7) cells. The CAP system was capable of rapid isolation and identification of CTCs without the requirement for pretreatment of blood samples. Finally, the CAP system was tested to evaluate the detection efficiency of CTCs in the blood samples of breast cancer patients. The number of captured CTCs in only 1ml of blood varied from 6 to 10.
Full Text
http://www.sciencedirect.com/science/article/pii/S0021967314017853
DOI
10.1016/j.chroma.2014.11.027
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seung Il(김승일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/138588
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