망상직내피세포(網狀織內被細胞) 계통이 신장조직`아렐기 -'에 미치는 영향에 관한 실험적 연구

Abstract

[한글]

Experimental Studies on the Influences of R.E.S. Blockade on Allergic Changes in

the Kidney

Yoon Keun Cha

(Director Prof. I.S. Yun, M.D.)

Dept. of Path., School of Medicine, Yon Sei University

Since it has been postulated that reticulo-end-othelial system may give the

influences to allergic reaction. various investigation on R.E.S, have beconme as a

target subject to clarify the mechanism of allergic phenomenon.

Some investigators suggested that R.E.S. are closely related to the production of

antibody, as they found the inhibition of anaphylactic shock in R.E.S. blockade

animal, experimentally. Yet enhancement of anaphylactic shock in R.E.S. blockade

with small amount of India ink to the experimental animals is reported the

existence of inhibitory substance against anaphylactic shock in the animal serum of

R.E.S blockade.

Marked inhibition of allergic changes in various organ of R.E.S. blockaded

animal, in both small and large amount of India ink administrantion, is reported.

This experiment has been planned to study the influences of R.E.S. upon the

allergic changes in the kidney.

Experimental Material and Methods

Normal adult rabbits weighing around 2 kilograms were used for this experiment

and divided into 6 groups.

For sensitizing, 1cc. of normal horse seum per kg. of body weight was injected

subcutaeously 4times, and 0.1cc. of normal horse serum per kg. of body weight was

reinjected for producing allergic changes in tissue.

For blockading the R.E.S., 0.43% of India ink in saline soultion was injected

intravenousy.

1) Control

As a control experiment, allergic changes were observed in rabbits in which

normal serum was injected subcutaneously four times for sensitizing, and for

reinjection 0.1 cc. per kg. of body weight of normal horse serum was given locally

in kidney through the renal artery, two weeks later. The allergic changes in the

kidkey were observed.

2) Single injection of India ink solution 5cc. of India ink solution was injected

intravenously 24 hours before sensitization, and then produced allergy and

observed.

3) Plural injection of india ink solution 5cc. of india ink solution was injected

8 times intravenously with intervals of 24 hours. Sensitization began 24 hours

later the last injection of India ink solution. The allergic changes was produced

in kidney and observed.

4) Injection of Serum from Normal rabbits Serum from normal rabbit blood was

injected intravenously in amount of 1.5cc. per kg. of body weight, and then

produced allergic phenomenon by usual method.

5) Injection of the serum from the rabbit, to which single injection of 5cc. of

India ink solution was given.

The serum from rabbits, to which single 5cc. injection of India ink solution was

made, was given intravenously, in amount of 1.5cc. per body weight, 24 hours before

sensitization. And allergic changes were produced and observed.

6) Injection of the serum from the rabbit, to which plural injection of 5cc. of

India ink solution was given.

The serum from rabbits, to which 5cc. of India ink solution had been injected 8

times, was given in amount of 1.5cc. per kg of body weight of experimental animals,

24 hours before sensitization, and produced allergic phenomenon in the kidney.

Conclusion

Studies on the influences of R.E.S. blockade upon the allergic changes in the

kidney are reported.

The allergic changes in the R.E.S. blockade with small amount of india ink were

slightly enhanced than normal control groups.

The allergic phenomenon in the R.E.S. blockade with the large amount of India ink

were much less than in those of the normal group.

The allergic changes in the group of normal rabbit serum injected were slightly

elevated and accelerated than in those of normally produced allergic group, and

samely enhanced but not accelerated allergic phenomenon in the kidney are observed

from the rabbits to which the serum from R.E.S. blockade with small amount of india

ink solution was given.

The injection of serum, obtained from the R.E.S. blockade rabbits by multiple

injected of india ink solution, inhibited allergic changes in the kindney of

experimental rabbits.

[영문]

Since it has been postulated that reticulo-end-othelial system may give the influences to allergic reaction. various investigation on R.E.S, have beconme as a target subject to clarify the mechanism of allergic phenomenon.

Some investigators suggested that R.E.S. are closely related to the production of antibody, as they found the inhibition of anaphylactic shock in R.E.S. blockade animal, experimentally. Yet enhancement of anaphylactic shock in R.E.S. blockade

with small amount of India ink to the experimental animals is reported the existence of inhibitory substance against anaphylactic shock in the animal serum of R.E.S blockade.

Marked inhibition of allergic changes in various organ of R.E.S. blockaded animal, in both small and large amount of India ink administrantion, is reported.

This experiment has been planned to study the influences of R.E.S. upon the allergic changes in the kidney.

Experimental Material and Methods

Normal adult rabbits weighing around 2 kilograms were used for this experiment and divided into 6 groups.

For sensitizing, 1cc. of normal horse seum per kg. of body weight was injected subcutaeously 4times, and 0.1cc. of normal horse serum per kg. of body weight was reinjected for producing allergic changes in tissue.

For blockading the R.E.S., 0.43% of India ink in saline soultion was injected intravenousy.

1) Control

As a control experiment, allergic changes were observed in rabbits in which normal serum was injected subcutaneously four times for sensitizing, and for reinjection 0.1 cc. per kg. of body weight of normal horse serum was given locally in kidney through the renal artery, two weeks later. The allergic changes in the kidkey were observed.

2) Single injection of India ink solution 5cc. of India ink solution was injected intravenously 24 hours before sensitization, and then produced allergy and observed.

3) Plural injection of india ink solution 5cc. of india ink solution was injected 8 times intravenously with intervals of 24 hours. Sensitization began 24 hours later the last injection of India ink solution. The allergic changes was produced

in kidney and observed.

4) Injection of Serum from Normal rabbits Serum from normal rabbit blood was injected intravenously in amount of 1.5cc. per kg. of body weight, and then produced allergic phenomenon by usual method.

5) Injection of the serum from the rabbit, to which single injection of 5cc. of India ink solution was given.

The serum from rabbits, to which single 5cc. injection of India ink solution was made, was given intravenously, in amount of 1.5cc. per body weight, 24 hours before sensitization. And allergic changes were produced and observed.

6) Injection of the serum from the rabbit, to which plural injection of 5cc. of India ink solution was given.

The serum from rabbits, to which 5cc. of India ink solution had been injected 8 times, was given in amount of 1.5cc. per kg of body weight of experimental animals, 24 hours before sensitization, and produced allergic phenomenon in the kidney.

Conclusion

Studies on the influences of R.E.S. blockade upon the allergic changes in the kidney are reported.

The allergic changes in the R.E.S. blockade with small amount of india ink were slightly enhanced than normal control groups.

The allergic phenomenon in the R.E.S. blockade with the large amount of India ink were much less than in those of the normal group.

The allergic changes in the group of normal rabbit serum injected were slightly elevated and accelerated than in those of normally produced allergic group, and samely enhanced but not accelerated allergic phenomenon in the kidney are observed

from the rabbits to which the serum from R.E.S. blockade with small amount of india ink solution was given.

The injection of serum, obtained from the R.E.S. blockade rabbits by multiple injected of india ink solution, inhibited allergic changes in the kindney of experimental rabbits.